Wang Yang-Yang, Zhu Rui-Lou, Chang En-Qiang, Liu Xiao-Zhuan, Wang Guang-Zhi, Li Ning-Tao, Zhang Wei, Zhou Jun, Sun Ming-Yang, Zou Xin, Hao Jie, Zhang Jia-Qiang
Department of Anesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, 450003, Henan, China.
Center for Clinical Single Cell Biomedicine, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, 450003, Henan, China.
Mol Med. 2025 May 7;31(1):178. doi: 10.1186/s10020-025-01213-6.
The immune system of patients undergoing major surgery usually has obvious immune responses during the perioperative period, and the patient's immune status would affect the patient's prognosis. In this study single-cell sequencing technology was used to investigate the effect of surgery/anesthesia on peripheral blood mononuclear cells (PBMCs) in depth during the perioperative period.
We performed an in-depth analysis of our previously published data, which included a total of 4 patients were recruited in this study. Their peripheral blood samples were collected pre operation, 0, 24, and 48 h post operation, and then PBMCs were extracted, followed by single cell sequencing. The results of sequencing were analyzed with R packages seurat and scSTAR. Finally, RT-PCR technology was used to verify the expression of key genes in monocyte.
The ratio of CD4 and CD8 T cells and Tregs showed little change, and the function of CD4 and CD8 T cells recovered soon. The function of Treg had not been restored 48 h post operation. Non-classical monocyte was impressed after surgery and showed no recovery trend within 48 h. Similar to scRNA-seq, the expression levels of MDM2 and SESN1 in patients with tumor increased significantly after surgery.
Surgery/anesthesia had little effect on CD4 and CD8 T cells, and continued to affect the functional changes of Treg. It had more impact on monocytes, which may cause them to promote tumor development to a certain extent.
接受大手术的患者免疫系统在围手术期通常会有明显的免疫反应，患者的免疫状态会影响其预后。在本研究中，采用单细胞测序技术深入研究手术/麻醉对围手术期外周血单个核细胞（PBMCs）的影响。
我们对之前发表的数据进行了深入分析，本研究共纳入4例患者。在术前、术后0、24和48小时采集他们的外周血样本，然后提取PBMCs，接着进行单细胞测序。测序结果用R包seurat和scSTAR进行分析。最后，用RT-PCR技术验证单核细胞中关键基因的表达。
CD4和CD8 T细胞以及调节性T细胞（Tregs）的比例变化不大，CD4和CD8 T细胞的功能很快恢复。术后48小时Treg的功能尚未恢复。手术后非经典单核细胞受到影响，在48小时内没有恢复趋势。与单细胞RNA测序（scRNA-seq）类似，肿瘤患者术后MDM2和SESN1的表达水平显著升高。
手术/麻醉对CD4和CD8 T细胞影响不大，但持续影响Treg的功能变化。对单核细胞影响更大，这可能在一定程度上导致它们促进肿瘤发展。
