Kamel Lamiaa Mahmoud, Shawky Nagwa Mohamed, Jouda Amal A, Ibrahim Amany Mohamed, Mahboub Heba H, Mohammed Sherif Y
Clinical Pathology Department, Faculty of Human Medicine, Zagazig University, Zagazig, Egypt.
Gastroenterology hepatology and infectious diseases Department, Faculty of Human Medicine, Zagazig University, Zagazig, Egypt.
Sci Rep. 2025 May 8;15(1):16052. doi: 10.1038/s41598-025-99037-1.
Hepatocellular carcinoma (HCC) is a significant health concern, ranking as the fourth most common cancer in Egypt and the sixth globally. Research has identified over 300 single nucleotide polymorphisms (SNPs) in the ghrelin gene, with four of these SNPs being associated with pathogenicity. The current work is a pioneer attempt to evaluate the role of Ghrelin gene polymorphism as a risk factor for progression of chronic viral hepatitis to cirrhosis and hepatocellular carcinoma in Egyptian patients. This study was carried out on 80 cases and were allocated into four groups: Group I: apparently healthy individuals, Group II: patients with chronic viral hepatitis, Group III: patients with post-hepatitic cirrhosis, and Group IV: patients with viral hepatitis-related HCC. Serum Ghrelin was measured by ELISA Kit. Molecular detection of Ghrelin rs34911341 and rs696217 were assessed using DNA sequencing. Outcomes showed that in terms of ghrelin gene polymorphism, every group under study had a GG rs34911341. The frequency of rs696217 genotype CA was statistically significantly higher in controls than in cirrhotic and HCC cases. When cirrhosis and HCC cases were compared to controls and chronic active hepatitis cases, the serum ghrelin level decreased statistically significantly. Taken together, there was no relation of ghrelin gene polymorphism in rs34911341 with progression of chronic active hepatitis. Moreover, the frequency of rs696217 genotype CA was increased in controls compared to patients with chronic viral hepatitis and patients with viral hepatitis-related HCC. Compared to controls, liver disease patients had lower serum Ghrelin levels.
肝细胞癌（HCC）是一个重大的健康问题，在埃及是第四大常见癌症，在全球是第六大常见癌症。研究已在胃饥饿素基因中鉴定出300多个单核苷酸多态性（SNP），其中四个SNP与致病性相关。目前的工作是一项开创性的尝试，旨在评估胃饥饿素基因多态性作为埃及患者慢性病毒性肝炎进展为肝硬化和肝细胞癌的危险因素的作用。本研究对80例患者进行，分为四组：第一组：明显健康的个体；第二组：慢性病毒性肝炎患者；第三组：肝炎后肝硬化患者；第四组：病毒性肝炎相关肝细胞癌患者。采用酶联免疫吸附测定试剂盒检测血清胃饥饿素。使用DNA测序评估胃饥饿素rs34911341和rs696217的分子检测。结果显示，就胃饥饿素基因多态性而言，每个研究组都有rs34911341的GG型。rs696217基因型CA的频率在对照组中在统计学上显著高于肝硬化和肝细胞癌病例。当将肝硬化和肝细胞癌病例与对照组和慢性活动性肝炎病例进行比较时，血清胃饥饿素水平在统计学上显著降低。综上所述，rs34911341中胃饥饿素基因多态性与慢性活动性肝炎的进展无关。此外，与慢性病毒性肝炎患者和病毒性肝炎相关肝细胞癌患者相比，对照组中rs696217基因型CA的频率增加。与对照组相比，肝病患者的血清胃饥饿素水平较低。
