Wijaya Shoma A, Pujianto Dwi A, Prijanti Ani R, Widyantoro Bambang
Doctoral Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
Department of Cardiology and Vascular Medicine, National Cardiovascular Center Harapan Kita, Jakarta, Indonesia.
Narra J. 2025 Apr;5(1):e1759. doi: 10.52225/narra.v5i1.1759. Epub 2025 Jan 23.
The therapeutic approach to hypertension often varies across racial and ethnic groups; however, antihypertensive treatments have not yet been tailored to account for these variations in Indonesia, a country with diverse racial and ethnic groups. In addition, microRNA-133 (miR-133) and microRNA-155 (miR-155) play critical roles in cardiac muscle homeostasis and inflammatory responses, but their specific functions in hypertension remain unclear. The aim of this study was to investigate the correlation between circulating miR-133 and miR-155 levels and angiotensin II (ANG-II) levels in hypertensive patients from Melanesian and non-Melanesian populations in Indonesia. A cross-sectional study was conducted in Jayapura, Indonesia among Melanesian and non- Melanesian hypertensive patients. The levels of ANG-II were quantified using sandwich ELISA, while the relative expression of miR-133 and miR-155 levels were measured by real-time PCR. Differences between the two groups were assessed using the Mann- Whitney test, and correlations between miR and ANG-II levels were determined using the Spearman correlation test. The relative expression levels of miR-133 and miR-155 in the Melanesian group were significantly higher than in the non-Melanesian group; 6.94-fold (3.85 vs 0.55) and 2.1-fold higher (0.19 vs 0.09), respectively. MiR-133 had a moderate negative correlation with ANG-II in both Melanesian ( = -0.538; < 0.001) and non- Melanesian ( = -0.649; < 0.001). However, miR-155 had no significant correlation with ANG-II levels in either the Melanesian group ( = 0.551) or non-Melanesian group ( = 0.159). This study highlights that miR-133 levels are significantly correlated with ANG- II concentrations in both Melanesian and non-Melanesian hypertensive patients, suggesting that miR-133 may play a regulatory role in the ANG-II pathway. These findings provide insights into the potential of miR-133 as a biomarker for hypertension management in diverse populations.
高血压的治疗方法在不同种族和民族群体中往往存在差异；然而，在印度尼西亚这个拥有多种族和民族群体的国家，抗高血压治疗尚未针对这些差异进行调整。此外，微小RNA - 133（miR - 133）和微小RNA - 155（miR - 155）在心肌稳态和炎症反应中发挥关键作用，但其在高血压中的具体功能仍不清楚。本研究的目的是调查印度尼西亚美拉尼西亚和非美拉尼西亚人群中高血压患者循环miR - 133和miR - 155水平与血管紧张素II（ANG - II）水平之间的相关性。在印度尼西亚查亚普拉对美拉尼西亚和非美拉尼西亚高血压患者进行了一项横断面研究。使用夹心ELISA法对ANG - II水平进行定量，同时通过实时PCR法测量miR - 133和miR - 155水平的相对表达。两组之间的差异采用曼 - 惠特尼检验进行评估，miR与ANG - II水平之间的相关性采用斯皮尔曼相关性检验确定。美拉尼西亚组中miR - 133和miR - 155的相对表达水平显著高于非美拉尼西亚组；分别高6.94倍（3.85对0.55）和2.1倍（0.19对0.09）。在美拉尼西亚（r = -0.538；P < 0.001）和非美拉尼西亚（r = -0.649；P < 0.001）人群中，miR - 133与ANG - II均呈中度负相关。然而，在美拉尼西亚组（r = 0.551）或非美拉尼西亚组（r = 0.159）中，miR - 155与ANG - II水平均无显著相关性。本研究强调，在美拉尼西亚和非美拉尼西亚高血压患者中，miR - 133水平均与ANG - II浓度显著相关，这表明miR - 133可能在ANG - II途径中发挥调节作用。这些发现为miR - 133作为不同人群高血压管理生物标志物的潜力提供了见解。
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