Jones Zachary D, Jin 金佳鸿 Jiahong, Zhu Yong, Hu 胡新华 Xin-Hua
Department of Physics, East Carolina University, Greenville, NC 27858, USA.
Institute for Advanced Optics and School of Physics & Electronic Science, Hunan Institute of Science and Technology, Yueyang, Hunan 414006, China.
IEEE Trans Instrum Meas. 2024;73. doi: 10.1109/tim.2024.3373072. Epub 2024 Mar 12.
Multiparameter spectrophotometry (MPS) provides a powerful and environmental-friendly tool for characterization of turbid materials including foods without additives. Existing methods of MPS, however, lack either modeling accuracy or capacity for translation into ease-of-use instrument. We have developed a novel method of MPS to rapidly retrieve the spectral data of absorption coefficient, scattering coefficient and anisotropy factor within the framework of radiative transfer (RT) theory without using integrating spheres. To demonstrate the feasibility of our MPS method for food analysis, the RT parameters have been determined between 500 and 1000 nm for 4 types of milk samples and skim milk samples spiked with either Lactobacillus acidophilus (L. acid.) or DH5α Escherichia coli (E. coli) bacteria. The spectral data has been shown to agree with the published results on milk composition. We have also found the threshold of number density for detection of E. coli in skim milk to be about 2×10 (mm). These results show clearly the potential of the presented method for development of accurate, ease-of-use and cost-effective instrument for routine analysis of turbid materials by MPS.
多参数分光光度法（MPS）为表征包括无添加剂食品在内的浑浊物质提供了一种强大且环保的工具。然而，现有的MPS方法要么缺乏建模精度，要么缺乏转化为易于使用的仪器的能力。我们开发了一种新颖的MPS方法，可在不使用积分球的情况下，在辐射传输（RT）理论框架内快速获取吸收系数、散射系数和各向异性因子的光谱数据。为证明我们的MPS方法用于食品分析的可行性，已测定了4种牛奶样品以及添加嗜酸乳杆菌（嗜酸乳杆菌）或DH5α大肠杆菌（大肠杆菌）的脱脂牛奶样品在500至1000纳米之间的RT参数。光谱数据已被证明与已发表的牛奶成分结果一致。我们还发现脱脂牛奶中检测大肠杆菌的数密度阈值约为2×10（毫米）。这些结果清楚地表明了所提出的方法在开发用于通过MPS对浑浊物质进行常规分析的准确、易用且经济高效的仪器方面的潜力。
