Castellot J J, Hoover R L, Harper P A, Karnovsky M J
Am J Pathol. 1985 Sep;120(3):427-35.
The regulation of cell growth in the kidney glomerulus plays a key role in many physiologic and pathologic processes. In this communication, the authors examine the possible role of heparin-like species as inhibitors of mesangial-cell proliferation. Heparin profoundly inhibited the growth of cultured mesangial cells in a dose-dependent manner, with an ED50 = 5-10 micrograms/ml. The antiproliferative activity of heparin was reversible and specific for mesangial cells as the target cell in the glomerulus. Heparin was much more effective than other glycosaminoglycans. Cultured glomerular epithelial cells were found to secrete both stimulators and inhibitors of mesangial-cell growth. Approximately half of the inhibitory activity was destroyed by a highly purified heparinase; the other half was sensitive to trypsin. Approximately 80% of the mitogenic activity was protease-sensitive. These results suggest that heparin and glomerular epithelial cells may participate in mesangial-cell growth regulation.
肾小球中细胞生长的调节在许多生理和病理过程中起着关键作用。在本通讯中，作者研究了类肝素物质作为系膜细胞增殖抑制剂的可能作用。肝素以剂量依赖的方式显著抑制培养的系膜细胞生长，半数有效剂量（ED50）为5 - 10微克/毫升。肝素的抗增殖活性是可逆的，并且对肾小球中的靶细胞系膜细胞具有特异性。肝素比其他糖胺聚糖更有效。发现培养的肾小球上皮细胞既能分泌系膜细胞生长的刺激物，也能分泌抑制剂。大约一半的抑制活性被高度纯化的肝素酶破坏；另一半对胰蛋白酶敏感。大约80%的促有丝分裂活性对蛋白酶敏感。这些结果表明，肝素和肾小球上皮细胞可能参与系膜细胞生长的调节。
