参与对坦布苏病毒固有免疫反应的模式识别受体的鉴定。
Identification of pattern recognition receptors participating in the innate immune response to Tembusu virus.
作者信息
Huang Jingjing, Hu Xiaoyang, Peng Duo, Li Qiong, Song Yinuo, Zhang Dabing
机构信息
National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, People's Republic of China.
出版信息
Arch Virol. 2025 May 15;170(6):126. doi: 10.1007/s00705-025-06307-4.
The pattern recognition receptors (PRRs) participating in the innate immune response to Tembusu virus (TMUV) were investigated using duck embryo fibroblasts (DEFs). TMUV infection induced significant upregulation of RIG-I and MDA5 and strong IFN-α, IFN-β, IL-6, and Mx responses. Overexpression of RIG-I or MDA5 resulted in increased IFN-α, IFN-β, IL-6, and Mx expression and decreased TMUV replication. Conversely, knockdown of RIG-I or MDA5 resulted in decreased IFN-α, IFN-β, IL-6, and Mx expression and increased TMUV replication. These findings demonstrate that RIG-I and MDA5 are essential sensors in the innate immune defense against TMUV, orchestrating cytokine production and restricting viral replication.
利用鸭胚成纤维细胞（DEFs）研究了参与对坦布苏病毒（TMUV）固有免疫反应的模式识别受体（PRRs）。TMUV感染诱导RIG-I和MDA5显著上调，并引发强烈的IFN-α、IFN-β、IL-6和Mx反应。RIG-I或MDA5的过表达导致IFN-α、IFN-β、IL-6和Mx表达增加，TMUV复制减少。相反，RIG-I或MDA5的敲低导致IFN-α、IFN-β、IL-6和Mx表达减少，TMUV复制增加。这些发现表明，RIG-I和MDA5是针对TMUV固有免疫防御中的关键传感器，协调细胞因子产生并限制病毒复制。
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