Liu Zike, Ye Baolin, Ye Haoxiang, Zhong Qing, Kong Jiecheng, Zhou Xianxi, Ma Chunmei, Liu Aijun
Department of Anatomy, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, PR China.
Department of Histology and Embryology, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, PR China.
Int Immunopharmacol. 2025 Jun 17;158:114847. doi: 10.1016/j.intimp.2025.114847. Epub 2025 May 15.
Hair follicle stem cells (HFSCs) can quickly activate and migrate to the wound site, differentiating into epidermal stem cells to facilitate early epithelialization. During the wound healing process, microRNAs (miRNAs) function coordinately. Chinese medicine borneol is derived from the Cinnamomum camphora plant, and its principal component, L-borneol, is renowned for its potential in facilitating skin wound healing. However, it remains unclear whether L-borneol can stimulate HFSCs to differentiate into epidermal cells or whether miRNAs are involved in this process. In the current study, HFSCs were isolated from the vibrissae of rats and identified based on the expression of CD34, Integrin-β1 and keratin type 1 cytoskeletal 15(CK15). We observed that stimulation with L-borneol significantly increased the differentiation marker K14 in HFSCs, suggesting that L-borneol could promote the differentiation of HFSCs into basal layer cells. On this basis, we transfected and confirmed that rno-miR-127 inhibitor could promote the differentiation of HFSCs. Furthermore, we demonstrated that PODXL2 is a target gene of rno-miR-127 through dual-luciferase reporter assays and confirmed that the rno-miR-127 mimic could inhibit the expression of PODXL2. To further elucidate the targeting relationship, we constructed the siPODXL2 fragment using siRNA technology, demonstrating that reducing PODXL2 expression can inhibit the differentiation of HFSCs into basal layer cells. Finally, a rat full-thickness skin defect model illustrated L-borneol-mediated negative regulation of PODXL2 by rno-miR-127, promoting skin injury repair through HFSCs.
毛囊干细胞（HFSCs）能够迅速激活并迁移至伤口部位，分化为表皮干细胞以促进早期上皮形成。在伤口愈合过程中，微小RNA（miRNAs）发挥协同作用。中药冰片源自樟科植物樟，其主要成分L-冰片以促进皮肤伤口愈合的潜力而闻名。然而，L-冰片是否能刺激HFSCs分化为表皮细胞，以及miRNAs是否参与这一过程仍不清楚。在本研究中，从大鼠触须中分离出HFSCs，并根据CD34、整合素-β1和角蛋白1细胞骨架15（CK15）的表达进行鉴定。我们观察到，L-冰片刺激显著增加了HFSCs中分化标志物K14的表达，表明L-冰片可促进HFSCs向基底层细胞分化。在此基础上，我们转染并证实rno-miR-127抑制剂可促进HFSCs的分化。此外，通过双荧光素酶报告基因检测，我们证明PODXL2是rno-miR-127的靶基因，并证实rno-miR-127模拟物可抑制PODXL2的表达。为进一步阐明靶向关系，我们利用小干扰RNA（siRNA）技术构建了siPODXL2片段，证明降低PODXL2表达可抑制HFSCs向基底层细胞的分化。最后，大鼠全层皮肤缺损模型表明，rno-miR-127通过L-冰片介导对PODXL2的负调控，促进HFSCs介导的皮肤损伤修复。
