Naga Marwa Samir, Kamoun Elbadawy Abdel Aziz, Moaty Maha Abdel, Ghareeb Ahmed Zaki, El Din Mona Mohy, Omar Samia Soliman Abdel Rehim
Dental Biomaterials Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
Department of Chemistry, College of Science, King Faisal University, Al-Ahsa 31982, Saudi Arabia; Polymeric Materials Research Department, Advanced Technology and New Materials Research Institute (ATNMRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg Al-Arab 21934, Alexandria, Egypt.
Int Dent J. 2025 May 15;75(4):100828. doi: 10.1016/j.identj.2025.04.008.
INTRODUCTION: Dentin regeneration is pivotal to preserve tooth vitality. This study aims to evaluate, histologically, the dentine regenerative potential of a novel injectable boron-doped, mesoporous, bioactive glass nanoparticle (BMBGNPs) loaded alginate hydrogel in dogs METHODS: The formulation and optimisation of the novel alginate/BMBG NPs (20 wt. %) loaded composite hydrogel were performed. Next, 66 teeth of 3 dogs were allocated into 3 groups (each including 22 teeth) according to post-operative follow-up period: group I: 2 weeks, group II: 4 weeks, and group III: 8 weeks. Each group was further subdivided according to pulpotomy filling material into two subgroups, with subgroup 1 (alginate/BMBGNPs (20 wt. %) loaded hydrogel) and subgroup 2 mineral trioxide aggregate (MTA). Pulp chambers were mechanically exposed through class V cavities. A complete pulpotomy was executed. The tested materials were positioned on the radicular pulp and finally covered with resin composite restorations. One dog was sacrificed after 2, 4, and 8 weeks. Teeth were prepared for histological evaluation assessing inflammatory cell response, pulp tissue organisation, and dentin bridge formation. The Mann-Whitney U test was employed to evaluate the scores of histological parameters between tested materials (P ≤ .05). RESULTS: Alginate/BMBG NPs (20 wt. %) loaded hydrogel showed normal pulp configuration at 2 and 4 weeks, which was enhanced after 8 weeks (P ≤ .05). Moderate inflammatory reaction was noted at 2 weeks, which was improved after 4 and 8 weeks (P ≤ .05). MTA group demonstrated less favourable pulpal response and inflammatory reaction with a statistically significant difference across all observational periods (P ≤ .05). After 8 weeks all teeth in group 1 exhibited the thickest dentin bridge (P ≤ .05). CONCLUSIONS: Alginate/BMBG NPs (20 wt. %) loaded hydrogel offers the promise of regenerating dentin and maintaining pulp vitality reaching the desired level as an alternative to MTA. CLINICAL RELEVANCE: Alginate/BMBG NP loaded hydrogel is an alternative, reliable option for vital pulp therapy.
引言：牙本质再生对于维持牙齿活力至关重要。本研究旨在通过组织学方法评估一种新型可注射的负载硼掺杂介孔生物活性玻璃纳米颗粒（BMBGNPs）的藻酸盐水凝胶在犬类中的牙本质再生潜力。方法：对新型藻酸盐/BMBG NPs（20重量%）负载复合水凝胶进行配方设计和优化。接下来，根据术后随访时间将3只犬的66颗牙齿分为3组（每组22颗牙齿）：第一组：2周，第二组：4周，第三组：8周。每组再根据牙髓切断术填充材料进一步细分为两个亚组，亚组1（负载藻酸盐/BMBGNPs（20重量%）的水凝胶）和亚组2矿物三氧化物凝聚体（MTA）。通过V类洞机械暴露牙髓腔。进行完整的牙髓切断术。将测试材料置于根髓上，最后用树脂复合材料修复体覆盖。在2周、4周和8周后分别处死1只犬。制备牙齿用于组织学评估，评估炎症细胞反应、牙髓组织结构和牙本质桥形成。采用曼-惠特尼U检验评估测试材料之间组织学参数的得分（P≤0.05）。结果：负载藻酸盐/BMBG NPs（20重量%）的水凝胶在2周和4周时显示牙髓结构正常，8周后有所改善（P≤0.05）。2周时观察到中度炎症反应，4周和8周后有所改善（P≤0.05）。MTA组在所有观察期内牙髓反应和炎症反应均较差，差异有统计学意义（P≤0.05）。8周后，第一组所有牙齿均表现出最厚的牙本质桥（P≤0.05）。结论：负载藻酸盐/BMBG NPs（20重量%）的水凝胶有望再生牙本质并维持牙髓活力，达到与MTA相当的理想水平。临床意义：负载藻酸盐/BMBG NP的水凝胶是一种用于活髓治疗的可靠替代选择。
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