Gospodarska Emilia, Dastidar Ranjini Ghosh, Jaroslawska Julia, Rybiński Maciej, Raczyk Marianna, Tokarczyk-Malesa Kornelia, Romaszko Jerzy, Carlberg Carsten
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, ul. Trylińskiego 18, 10-683, Olsztyn, Poland.
Department of Family Medicine and Infectious Diseases, School of Medicine, Collegium Medicum, University of Warmia and Mazury, Olsztyn, Poland.
Sci Rep. 2025 May 19;15(1):17334. doi: 10.1038/s41598-025-02495-w.
The VitDPAS study (NCT06104111) was designed as a medical experiment to assess the in vivo effects of vitamin D on immune responses. This study enrolled 45 healthy individuals from Olsztyn, Poland, who received a body weight-adjusted bolus dose of vitamin D (1,000 IU/kg). Transcriptome-wide differential gene expression analysis of peripheral blood mononuclear cells, collected before and 24 h after supplementation, identified 758 significantly responsive genes (p < 0.05). By correlating individual gene expression changes with alterations in vitamin D status, participants were categorized into three response groups: 17 high responders, 19 mid responders, and 9 low responders. A comparative analysis with the VitDHiD study (NCT03537027), conducted on a Finnish cohort of 25 healthy participants, revealed 232 overlapping target genes, enabling an integrated assessment of vitamin D responsiveness across all 70 individuals. Applying a more stringent statistical threshold (false discovery rate < 0.05) highlighted 26 shared target genes, demonstrating a consistent in vivo response to vitamin D across both cohorts. The modulation of inflammatory processes, mediated primarily via tumor necrosis factor and nuclear factor κB signaling pathways, emerged as a shared effect, highlightening the immunomodulatory potential of vitamin D as a key function of the vitamin in healthy individuals.
维生素D对免疫反应的体内效应研究（VitDPAS研究，NCT06104111）被设计为一项医学实验，以评估维生素D对免疫反应的体内效应。该研究招募了来自波兰奥尔什丁的45名健康个体，他们接受了根据体重调整的大剂量维生素D（1000 IU/kg）。对补充前和补充后24小时采集的外周血单核细胞进行全转录组差异基因表达分析，确定了758个显著反应基因（p<0.05）。通过将个体基因表达变化与维生素D状态的改变相关联，参与者被分为三个反应组：17名高反应者、19名中反应者和9名低反应者。对芬兰25名健康参与者队列进行的维生素D高剂量研究（VitDHiD研究，NCT03537027）的比较分析揭示了232个重叠的靶基因，从而能够对所有70名个体的维生素D反应性进行综合评估。应用更严格的统计阈值（错误发现率<0.05）突出了26个共享靶基因，表明两个队列对维生素D的体内反应一致。主要通过肿瘤坏死因子和核因子κB信号通路介导的炎症过程调节是一种共同效应，突出了维生素D在健康个体中的关键功能——免疫调节潜力。
