Hu Meihua
Nanchang Normal University, College of Chemistry and Food Science, Nanchang 330032, China.
J AOAC Int. 2025 Sep 1;108(5):670-676. doi: 10.1093/jaoacint/qsaf054.
Basic orange 2, as an industrial dye, exhibits severe effects on human health and has been forbidden for use as a food additive in many countries. The practical use of a common pretreatment process for basic orange 2 in food might be limited owing to time-consuming, high consumption of solvents and weak selectivity. Therefore, the aim of this study was to validate a pretreatment technology for basic orange 2 based on simplicity, being rapid, good selectivity, high sensitivity, and inexpensiveness to ensure safety for people.
To develop a strategy for the extraction and enrichment of basic orange 2 in food matrixes by the specific magnetic molecularly imprinted polymers (MIPs) prior to the determination by high-performance liquid chromatography (HPLC).
The polymers with basic orange 2 as the template were deposited on the surface of ferroferric oxide nanoparticles (Fe3O4@PDA MIPs) through the self-assembly of dopamine in the weak alkaline solution. The Fe3O4@PDA MIPs as a dispersed solid phase extraction (SPE) sorbent were used for extracting and detecting basic orange 2 using HPLC. Parameters influencing extracting efficiencies were optimized, including the mass of MIPs, pH value, time for ultrasonication, elution solvent, and volume.
Under optimal experimental conditions, the detection linearity of basic orange 2 was in the range of 0.04-1.0 μg/mL with a correlation coefficient of 0.9991 and limit of detection (LOD) of 5.8 ng/mL. The recoveries from spiked samples were 73.3-90% with a RSD of 3.1-8.1% in food samples. Notably, the magnetic MIPs showed excellent reusability during three extraction procedures.
The Fe3O4@PDA MIPs prepared have great potential for purification and enrichment of basic orange 2 prior to its determination by HPLC in dried beancurd stick, yellowfish, chili powder, drinks, and wine.
The magnetic MIPs developed show good adsorption performance for basic orange 2 with a very low detection and excellent reusability. The assay was applied to detect basic orange 2 in food samples successfully.
碱性橙2作为一种工业染料，对人体健康有严重影响，在许多国家已被禁止用作食品添加剂。食品中碱性橙2常见预处理工艺的实际应用可能因耗时、溶剂消耗量大和选择性差而受到限制。因此，本研究的目的是验证一种基于简单、快速、选择性好、灵敏度高且成本低廉的碱性橙2预处理技术，以确保人们的安全。
开发一种策略，通过特定的磁性分子印迹聚合物（MIPs）在高效液相色谱（HPLC）测定之前对食品基质中的碱性橙2进行萃取和富集。
以碱性橙2为模板的聚合物通过多巴胺在弱碱性溶液中的自组装沉积在四氧化三铁纳米颗粒（Fe3O4@PDA MIPs）表面。Fe3O4@PDA MIPs作为分散固相萃取（SPE）吸附剂，用于通过HPLC萃取和检测碱性橙2。优化了影响萃取效率的参数，包括MIPs的质量、pH值、超声时间、洗脱溶剂和体积。
在最佳实验条件下，碱性橙2的检测线性范围为0.04 - 1.0μg/mL，相关系数为0.9991，检测限（LOD）为5.8 ng/mL。加标样品在食品样品中的回收率为73.3 - 90%，相对标准偏差（RSD）为3.1 - 8.1%。值得注意的是，磁性MIPs在三次萃取过程中表现出优异的可重复使用性。
所制备的Fe3O4@PDA MIPs在通过HPLC测定腐竹、黄鱼、辣椒粉、饮料和葡萄酒中的碱性橙2之前，具有对其进行纯化和富集的巨大潜力。
所开发的磁性MIPs对碱性橙2具有良好的吸附性能，检测限极低且可重复使用性优异。该方法成功应用于食品样品中碱性橙2的检测。
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