Stasheff S F, Bragdon A C, Wilson W A
Brain Res. 1985 Oct 7;344(2):296-302. doi: 10.1016/0006-8993(85)90807-8.
In this paper we present an in vitro model of epileptogenesis based on electrical stimulation rather than pharmacological or ionic manipulations. Hippocampal slices given a series of stimulus trains similar to those used in kindling exhibited 3 types of epileptiform activity in CA3: afterdischarges immediately following the trains; spontaneous bursts of multiple population spikes; and bursts triggered by single stimuli. The afterdischarges and spontaneous bursts may be comparable to those seen in vivo during kindling; also, the progression of these features in this model was similar to their progression during kindling. All epileptiform activities were long-lasting, persisting for up to 3.5 h following the last train. This stimulus train-induced population bursting should be valuable as an acute model of hippocampal epileptogenesis, and may also help elucidate hippocampal participation in the kindling process.
在本文中，我们提出了一种基于电刺激而非药理学或离子操作的癫痫发生体外模型。给予类似于点燃模型中使用的一系列刺激串的海马切片，在CA3区表现出3种癫痫样活动：刺激串后立即出现的后放电；多个群体峰电位的自发爆发；以及由单个刺激触发的爆发。后放电和自发爆发可能与点燃过程中体内观察到的情况相当；此外，这些特征在该模型中的进展与它们在点燃过程中的进展相似。所有癫痫样活动都持续时间较长，在最后一次刺激串后可持续长达3.5小时。这种刺激串诱导的群体爆发作为海马癫痫发生的急性模型应该是有价值的，并且可能也有助于阐明海马在点燃过程中的参与情况。
