肠炎沙门氏菌LA5饱和转座子突变体文库的构建与表征
Development and characterization of a saturated transposon mutant library of serovar Enteritidis LA5.
作者信息
Trotereau Jérôme, Huguet Anne-Sophie, Jouan Romain, Kempf Florent, Naquin Delphine, Schouler Catherine, Velge Philippe, Mergaert Peter, Virlogeux-Payant Isabelle
机构信息
INRAE, Université de Tours, ISP, Tours, Centre-Val de Loire, France.
Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell, Gif-sur-Yvette, Île-de-France, France.
出版信息
Microbiol Resour Announc. 2025 Jun 12;14(6):e0131924. doi: 10.1128/mra.01319-24. Epub 2025 May 27.
Abstract
We created and characterized a saturated Tn-seq mutant library in subsp. ser. Enteritidis strain LA5. The set of essential genes after culture in rich medium was determined. This functional genomics tool will aid in identifying the roles of chromosomal and plasmid virulence determinants.
摘要
我们构建并鉴定了肠炎沙门氏菌血清型肠炎亚种LA5菌株的饱和转座子测序（Tn-seq）突变文库。确定了在丰富培养基中培养后的必需基因集。这个功能基因组学工具将有助于确定染色体和质粒毒力决定因素的作用。
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