从猛犸象（Mammuthus primigenius）组织中分离脱氧核糖核酸并进行表征。

Isolation and characterization of deoxyribonucleic acid from tissue of the woolly mammoth, Mammuthus primigenius.

作者信息

Johnson P H, Olson C B, Goodman M

出版信息

Comp Biochem Physiol B. 1985;81(4):1045-51. doi: 10.1016/0305-0491(85)90112-9.

Abstract

DNA was isolated from tissue samples of several mammoth specimens, radiocarbon dated between 10,000 and 53,000 years old. The DNA was purified by chromatography on hydroxyapatite at 60 degrees C and was characterized as a heterogeneous population of fragments ranging in size from 3000 to 200 base pairs. Thermal denaturation analysis demonstrated that approximately 25% of the DNA had a base composition similar to Asian elephant DNA calculated as 36% G + C. Preliminary analysis by nucleic acid hybridization indicated that only a small fraction of DNA isolated from mammoth tissue (2-5%) was homologous to DNA of Asian elephant, a close living relative of the mammoth. Our results provide the first definitive isolation and characterization of DNA from ancient tissue and suggest a purification strategy that will lead to preparations of DNA from mammoth tissue significantly enriched in elephant-related DNA sequences.

摘要

从几个猛犸象标本的组织样本中分离出了DNA，这些样本经放射性碳测定年代在10000年至53000年之间。DNA在60摄氏度下通过羟基磷灰石色谱法进行纯化，其特征是片段大小在3000至200个碱基对之间的异质群体。热变性分析表明，大约25%的DNA碱基组成与亚洲象DNA相似，计算得出G + C含量为36%。核酸杂交的初步分析表明，从猛犸象组织中分离出的DNA中只有一小部分（2 - 5%）与猛犸象的近亲亚洲象的DNA同源。我们的结果首次明确地从古代组织中分离并鉴定了DNA，并提出了一种纯化策略，该策略将导致从猛犸象组织中制备出显著富含与大象相关DNA序列的DNA。