大鼠膀胱逼尿肌中产生的前列腺素概况：三磷酸腺苷和腺苷的作用。

Profile of prostaglandins generated in the detrusor muscle of rat urinary bladder: effects of adenosine triphosphate and adenosine.

作者信息

Kasakov L N, Vlaskovska M V

出版信息

Eur J Pharmacol. 1985 Jul 31;113(3):431-6. doi: 10.1016/0014-2999(85)90092-5.

Abstract

The generation and release of PGE2, PGF2 alpha, PGD2, TXB2 and 6-keto-PGF1 alpha in the rat detrusor muscle were studied by means of radioimmunoassays. The effect of ATP (0.1 mmol/1) and adenosine (0.1 mmol/1) on the content and profile of PGs in the incubation medium was investigated. It was found that PGE2 and 6-keto-PGF1 alpha accounted for more than 80% of the total PG activity. ATP increased the amounts of PGs in the incubation medium (percentage change of the control values, N = 6: PGE2 54.53 +/- 12.69, PGF2 alpha 31.01 +/- 8.82, PGD2 44.52 +/- 12.36, TXB2 17.29 +/- 10.45, 6-keto-PGF1 alpha 36.62 +/- 5.0) but did not change their profile. Adenosine had no effect on either content or profile of the PGs. The results suggest that ATP but ot adenosine may activate PG biosynthesis via P2-purinoceptor-mediated mechanisms.

摘要

采用放射免疫分析法研究了大鼠逼尿肌中前列腺素E2（PGE2）、前列腺素F2α（PGF2α）、前列腺素D2（PGD2）、血栓素B2（TXB2）和6-酮-前列腺素F1α（6-keto-PGF1α）的生成与释放。研究了三磷酸腺苷（ATP，0.1 mmol/L）和腺苷（0.1 mmol/L）对孵育培养基中前列腺素含量和谱型的影响。结果发现，PGE2和6-keto-PGF1α占前列腺素总活性的80%以上。ATP增加了孵育培养基中前列腺素的含量（相对于对照值的百分比变化，N = 6：PGE2为54.53±12.69，PGF2α为31.01±8.82，PGD2为44.52±12.36，TXB2为17.29±10.45，6-keto-PGF1α为36.62±5.0），但未改变其谱型。腺苷对前列腺素的含量和谱型均无影响。结果表明，ATP而非腺苷可能通过P2嘌呤受体介导的机制激活前列腺素的生物合成。