用[125I]TID对肌浆网进行疏水光标记
Hydrophobic photolabelling of sarcoplasmic reticulum with [125I]TID.
作者信息
Gutweniger H E, Montecucco C
出版信息
Int J Biochem. 1985;17(8):867-71. doi: 10.1016/0020-711x(85)90168-5.
PMID:4043508
Abstract
[125I]TID, a small photoreactive lipophylic reagent, was used to label intrinsic proteins of rabbit and rat sarcoplasmic reticulum membranes. A 160,000 glycoprotein, the Ca2+-ATPase and polypeptides of mol. wt 53-55,000, 30,000, 20,000 and 6000 dalton were labelled suggesting that these proteins are integral membrane components.
摘要
[125I]TID是一种小的光反应性亲脂性试剂，用于标记兔和大鼠肌浆网膜的内在蛋白。一种分子量为160,000的糖蛋白、Ca2+-ATP酶以及分子量为53 - 55,000、30,000、20,000和6000道尔顿的多肽被标记，这表明这些蛋白质是膜的组成成分。
Zotero 插件