Wang Tao, Deng MingZhu, Kalonda Mutamba Alpha, He XiaoRi, Bian Jing, Bian DuJun
Department of Pediatric, Second Xiangya Hospital of Central South University, Changsha, China.
Department of Radiology, Hunan Normal University, Changsha, China.
Curr Med Imaging. 2025;21:e15734056296733. doi: 10.2174/0115734056296733250514081236.
Intrauterine growth restriction (IUGR) is associated with long-term metabolic disturbances, including obesity. Changes in hepatic lipid metabolism and adipose tissue function, mediated by lipin-1 and lipin-2, may contribute to these outcomes.
This study aimed to investigate the correlation between lipin-1 in visceral adipose tissues (VATs) and lipin-2 in the liver. It also examined hepatic T1 values using T1 mapping in IUGR rats.
The objective of this study was to explore the metabolic mechanisms linking IUGR and adult obesity by analyzing molecular and imaging markers.
Pregnant rats were fed either a low-protein diet (10%) to induce IUGR or a normal-protein diet (21%) as a control. Male offspring underwent conventional magnetic resonance imaging and native T1 mapping using a 3.0 T whole-body MR scanner at days 21, 56, and 84 post-birth. Liver tissues and VATs were collected for analysis. Lipin-1 and lipin-2 expression levels were measured using Western blot and real-time quantitative PCR.
The IUGR group exhibited significantly higher mRNA and protein expression levels of lipin-1 and lipin-2 compared to the control group at days 21, 56, and 84 after birth. Additionally, the IUGR group demonstrated significantly higher hepatic T1 values than the control group at the corresponding time points. Positive correlations were observed between the protein and mRNA expression levels of lipin-1 and hepatic T1 values. Similarly, the protein and mRNA expression levels of lipin-2 were positively correlated with hepatic T1 values. All results were statistically significant (P<0.05).
The upregulation of lipin-1 and lipin-2 expressions was found to be linked to elevated hepatic T1 values, potentially contributing to adult obesity in IUGR rats.
宫内生长受限（IUGR）与包括肥胖在内的长期代谢紊乱有关。由脂素-1和脂素-2介导的肝脏脂质代谢和脂肪组织功能变化可能导致这些结果。
本研究旨在调查内脏脂肪组织（VATs）中脂素-1与肝脏中脂素-2之间的相关性。同时，利用T1映射技术检测IUGR大鼠的肝脏T1值。
本研究的目的是通过分析分子和成像标志物来探索将IUGR与成人肥胖联系起来的代谢机制。
给怀孕大鼠喂食低蛋白饮食（10%）以诱导IUGR，或喂食正常蛋白饮食（21%）作为对照。雄性后代在出生后第21、56和84天使用3.0 T全身磁共振成像仪进行传统磁共振成像和原生T1映射。收集肝脏组织和VATs进行分析。使用蛋白质印迹法和实时定量PCR测量脂素-1和脂素-2的表达水平。
在出生后第21、56和84天，IUGR组的脂素-1和脂素-2的mRNA和蛋白质表达水平显著高于对照组。此外，在相应时间点，IUGR组的肝脏T1值显著高于对照组。脂素-1的蛋白质和mRNA表达水平与肝脏T1值之间存在正相关。同样，脂素-2的蛋白质和mRNA表达水平与肝脏T1值呈正相关。所有结果均具有统计学意义（P<0.05）。
发现脂素-1和脂素-2表达上调与肝脏T1值升高有关，这可能导致IUGR大鼠成年后肥胖。
