Li Shijun, Luo Xiangwen, Zhang Yu, Zhang Zhanhong, Yang Xiao, Zhou Xuguo, Wang Xin, OuYang Xian, Qu Xiaoxin, Zhang Deyong, Zhang Songbai, Liu Yong
College of Plant Protection, Hunan Agricultural University, Changsha, China.
Key Laboratory of Pest Management of Horticultural Crop of Hunan Province, Institute of Plant Protection of Hunan Academy of Agricultural Science, Changsha, China.
Front Plant Sci. 2025 May 16;16:1551362. doi: 10.3389/fpls.2025.1551362. eCollection 2025.
The literature shows that vector-transmitted plant viruses mediate host volatile biosynthesis to attract vectors and repel non-vectors, benefiting plant-infecting viruses and enhancing their spread. In this study, pepper veinal yellows virus (PeVYV) and its encoded movement protein P4 were shown to mediate host volatile biosynthesis to co-attract its exclusive vector aphids and non-vector Q-whiteflies; both have been proven to have existing food competition. The P4 protein enhances the biosynthesis of three volatiles and reduces the biosynthesis of one volatile involved in co-attracting and co-repelling aphids and Q-whiteflies. Viral replication is enhanced significantly in plants co-fed on by aphids and Q-whiteflies compared with plants fed on individual insects. Viral replication is enhanced significantly in the early stage for plants fed on by Q-whiteflies while in the late stage for plants fed on by aphids. Trans-zeatin (tZ) biosynthesis was increased in plants fed on by aphids and Q-whiteflies, and tZ enhanced viral replication in these plants. These data suggest that the PeVYV P4 protein mediates host volatile biosynthesis to co-attract vectors and non-vectors, benefiting viral infection probably by enhancing tZ biosynthesis. Data in this study have broad implications regarding the ecological significance of whiteflies in various agricultural ecosystems where aphids are the key viral transmitters.
文献表明，媒介传播的植物病毒介导宿主挥发性物质的生物合成，以吸引媒介并排斥非媒介，这有利于感染植物的病毒并促进其传播。在本研究中，辣椒脉黄病毒（PeVYV）及其编码的运动蛋白P4被证明介导宿主挥发性物质的生物合成，以共同吸引其唯一的媒介蚜虫和非媒介Q型粉虱；两者已被证明存在食物竞争关系。P4蛋白增强了三种挥发性物质的生物合成，并减少了一种参与共同吸引和共同排斥蚜虫和Q型粉虱的挥发性物质的生物合成。与单独以昆虫为食的植物相比，在蚜虫和Q型粉虱共同取食的植物中病毒复制显著增强。对于以Q型粉虱为食的植物，病毒复制在早期显著增强，而对于以蚜虫为食的植物，病毒复制在后期显著增强。在蚜虫和Q型粉虱取食的植物中反式玉米素（tZ）的生物合成增加，并且tZ增强了这些植物中的病毒复制。这些数据表明，PeVYV P4蛋白介导宿主挥发性物质的生物合成以共同吸引媒介和非媒介，可能通过增强tZ生物合成而有利于病毒感染。本研究中的数据对于粉虱在蚜虫作为关键病毒传播者的各种农业生态系统中的生态意义具有广泛的启示。
