Kundu Somenath, Shit Basudev, Paria Samaresh, Barman Subhajit, Nasrin Aziza, Karan Putul, Hossain Maidul
Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, West Bengal, 721102, India.
J Fluoresc. 2025 Jun 2. doi: 10.1007/s10895-025-04385-w.
A sustainable, efficient, and eco-friendly approach was developed to synthesize silver nanoparticles using Euphorbia neriifolia leaf extract. The biosynthesized Euphorbia neriifolia silver nanoparticles (ENSNPs) were comprehensively characterized using several techniques, including absorbance spectroscopy, Fourier transform infrared spectroscopy, high-resolution transmission electron microscopy, field emission scanning electron microscopy, X-ray photoelectron spectroscopy, and dynamic light scattering. ENSNPs quenched the intrinsic fluorescence of human serum albumin (HSA) with a quenching constant of 1.10 ± 0.004 × 10 M at 298.15 K, and the Time-correlated single-photon counting experiment clearly indicated that the quenching mechanism is static. Moreover, synchronous spectroscopy, 3D fluorescence spectroscopy, and circular dichroism spectra further revealed that the microenvironment of amino acid residues in HSA was slightly changed after interaction with ENSNPs. The thermodynamic investigation and docking analysis collectively showed that HSA-ENSNPs complexation was driven by hydrogen bonding, pi-alkyl interactions, and Van der Waals forces, providing insight into the nature of the binding interactions and the stability of the complex.
开发了一种可持续、高效且环保的方法，利用大戟叶提取物合成银纳米颗粒。使用多种技术对生物合成的大戟叶银纳米颗粒（ENSNPs）进行了全面表征，包括吸收光谱、傅里叶变换红外光谱、高分辨率透射电子显微镜、场发射扫描电子显微镜、X射线光电子能谱和动态光散射。在298.15 K时，ENSNPs淬灭了人血清白蛋白（HSA）的固有荧光，淬灭常数为1.10±0.004×10 M，时间相关单光子计数实验清楚地表明淬灭机制是静态的。此外，同步光谱、三维荧光光谱和圆二色光谱进一步表明，HSA与ENSNPs相互作用后，其氨基酸残基的微环境发生了轻微变化。热力学研究和对接分析共同表明，HSA-ENSNPs的络合是由氢键、π-烷基相互作用和范德华力驱动的，这为结合相互作用的性质和络合物的稳定性提供了深入了解。
