Zhang Hui, Yu Qisheng, Guo Rui, Zhu Qing, Yu Jin, Zhang Zhaohui, Lan Lan, Tang Cheng, Yu Changqing, Zhang Bin
College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, 610041, China.
Center for Animal Disease Control and Prevention, Ganzi Tibetan Autonomous Prefectue, Kangding, 626000, China.
BMC Vet Res. 2025 Jun 3;21(1):398. doi: 10.1186/s12917-025-04848-z.
Bovine coronavirus (BCoV) is a major pathogen of bovine respiratory disease, causing respiratory and enteric infections in cattle and wild ruminants. It is responsible for economic losses and threatens the health and welfare of cattle industry.
In this study, a BCoV isolate, BCoV/SUWN/XHD-5, had cytopathogenic effects in Madin Darby bovine kidney (MDBK) cells and showed a high viral titer at 24 and 48 h post infection (hpi). Gene expression profiling using RNA sequencing and protein network mapping using the Tandem Mass Tag-based quantitative proteomics approach were performed in MDBK cells with BCoV infection at 24 and 48 hpi, respectively. Compared with mock-infected MDBK cells, 8,720 differentially expressed genes (DEGs) and 296 differentially expressed proteins (DEPs) were identified in BCoV infection at 24 hpi, whereas 5,838 DEGs and 747 DEPs were identified in BCoV infection at 48 hpi. Following GO annotation and KEGG enrichment analysis, most DEGs and DEPs were significantly enriched in metabolic pathways, endocytosis, ribosome and protein processing in endoplasmic reticulum, apoptosis and immune response. A correlation analysis of the proteome and transcriptome revealed that the up-regulated DEGs and DEPs were predominantly associated with metabolic pathways, apoptosis and the MAPK/TNF/Ras signaling pathway, whereas the down-regulated DEGs and DEPs were involved in complement and coagulation cascades and the Wnt signaling pathway. Importantly, BCoV decreases the mRNA and protein levels of complement component C3 in MDBK cells.
The findings of this study provide the first report of the integrative transcriptomic and proteomic analyses of BCoV infection in MDBK cells. It revealed a regulatory network for analyzing the mechanisms of BCoV-host interactions and provides valuable insights into the pathogenesis of BCoV.
牛冠状病毒（BCoV）是牛呼吸道疾病的主要病原体，可引起牛和野生反刍动物的呼吸道和肠道感染。它会造成经济损失，并威胁到养牛业的健康和福利。
在本研究中，一株BCoV分离株BCoV/SUWN/XHD-5在马迪达比牛肾（MDBK）细胞中具有细胞病变效应，并在感染后24小时和48小时（hpi）显示出高病毒滴度。分别在感染BCoV的MDBK细胞中于24 hpi和48 hpi进行了基于RNA测序的基因表达谱分析和基于串联质量标签的定量蛋白质组学方法的蛋白质网络映射。与 mock 感染的 MDBK 细胞相比，在24 hpi的BCoV感染中鉴定出8720个差异表达基因（DEG）和296个差异表达蛋白（DEP），而在48 hpi的BCoV感染中鉴定出5838个DEG和747个DEP。经过GO注释和KEGG富集分析，大多数DEG和DEP在代谢途径、内吞作用、核糖体和内质网中的蛋白质加工、细胞凋亡和免疫反应中显著富集。蛋白质组和转录组的相关性分析表明，上调的DEG和DEP主要与代谢途径、细胞凋亡和MAPK/TNF/Ras信号通路相关，而下调的DEG和DEP则参与补体和凝血级联反应以及Wnt信号通路。重要的是，BCoV降低了MDBK细胞中补体成分C3的mRNA和蛋白质水平。
本研究结果首次报道了对MDBK细胞中BCoV感染的综合转录组学和蛋白质组学分析。它揭示了一个用于分析BCoV-宿主相互作用机制的调控网络，并为BCoV的发病机制提供了有价值的见解。
