Huoy Laingshun, Nasirzadeh Leila, Phan Kongkea, Tieng Siteng, Sternberg-Lewerin Susanna, Bongcam-Rudloff Erik, Boqvist Sofia
Department of Bioengineering, Faculty of Engineering, Royal University of Phnom Penh, Russian Federation Boulevard, Teuk Laak 1, Toul Kork, 120404 Phnom Penh, Cambodia.
Department of Animal Biosciences, Swedish University of Agricultural Sciences, Ulls väg 26, 756-51 Uppsala, Sweden.
J Appl Microbiol. 2025 Jun 2;136(6). doi: 10.1093/jambio/lxaf137.
To determine the Salmonella serotype distribution, antimicrobial resistance profiles, and antimicrobial resistance genes (ARGs) in food samples obtained from local markets in a low-income urban setting and nearby farms in Cambodia.
One hundred and thirty-nine Salmonella isolates from various food sources were tested for antibiotic susceptibility using a panel of 12 antibiotics, and 81 selected Salmonella isolates were further sequenced for serotype distribution and ARG identification. The results showed that 71% (99/139) of the isolates exhibited resistance to at least one antibiotic, with 39% (39/99) classified as multidrug-resistant (MDR). The highest resistance was observed against azithromycin (37%), followed by oxytetracycline (35%). A total of 32 serotypes were identified, with the six most common being S. Corvallis (7%), S. Haifa (6%), S. Weltevreden (6%), S. Agona (5%), S. Kentucky (5%), and S. Livingstone (5%). A broad range of ARGs was observed across multiple antibiotic classes, including macrolides, aminoglycosides, tetracyclines, phenicols, fluoroquinolones, sulfonamide-trimethoprim, beta-lactams, and MDR genes.
The results highlight the potential role of fresh food products in the widespread dissemination of Salmonella strains resistant to multiple antibiotics.
确定从柬埔寨低收入城市地区当地市场和附近农场采集的食品样本中沙门氏菌的血清型分布、抗菌药物耐药谱及抗菌药物耐药基因（ARGs）。
使用一组12种抗生素对来自各种食物来源的139株沙门氏菌分离株进行抗生素敏感性测试，并对81株选定的沙门氏菌分离株进一步测序以确定血清型分布和ARGs。结果显示，71%（99/139）的分离株对至少一种抗生素耐药，其中39%（39/99）被归类为多重耐药（MDR）。观察到对阿奇霉素的耐药率最高（37%），其次是土霉素（35%）。共鉴定出32种血清型，六种最常见的血清型为科瓦利斯沙门氏菌（7%）、海法沙门氏菌（6%）、韦尔泰弗里登沙门氏菌（6%）、阿贡纳沙门氏菌（5%）、肯塔基沙门氏菌（5%）和利文斯通沙门氏菌（5%）。在多种抗生素类别中观察到广泛的ARGs，包括大环内酯类、氨基糖苷类、四环素类、酚类、氟喹诺酮类、磺胺甲恶唑、β-内酰胺类和MDR基因。
结果突出了新鲜食品在多重耐药沙门氏菌菌株广泛传播中的潜在作用。
