球形节杆菌细胞对氢化可的松转化过程中的氧化还原反应。

Redox reactions in hydrocortisone transformation by Arthrobacter globiformis cells.

作者信息

Arinbasarova A Y, Medentsev A G, Akimenko V K, Koshcheyenko K A, Skryabin G K

出版信息

J Steroid Biochem. 1985 Sep;23(3):307-12. doi: 10.1016/0022-4731(85)90409-1.

Abstract

Hydrocortisone and prednisolone transformation by Arthrobacter globiformis cells in aerobic and anaerobic conditions was studied. 3-Ketosteroid-1-en-dehydrogenase activity was shown to be the major factor regulating the direction of transformation. When it is high (aerobic conditions), the end products of hydrocortisone transformation are prednisolone or its 20 beta-hydroxy derivative. The latter is produced via 1-en-dehydrogenation, which is not a limiting stage of the process. Low 3-ketosteroid-1-en-dehydrogenase activity (in the presence of cyanide) or its complete inhibition (strictly anaerobic conditions) result in the direct reduction of 20-keto group of hydrocortisone.

摘要

研究了球形节杆菌细胞在需氧和厌氧条件下对氢化可的松和泼尼松龙的转化。结果表明，3-酮甾体-1-烯脱氢酶活性是调节转化方向的主要因素。当该酶活性较高时（需氧条件），氢化可的松转化的终产物是泼尼松龙或其20β-羟基衍生物。后者通过1-烯脱氢作用产生，而这并非该过程的限速阶段。3-酮甾体-1-烯脱氢酶活性较低（存在氰化物时）或其完全被抑制（严格厌氧条件）会导致氢化可的松的20-酮基直接还原。