Motsar Elena, Sheveleva Anna, Sharko Fedor, Petrova Kristina, Slobodova Natalia, Murataev Ramil, Mitrofanova Irina, Chirkov Sergei
Department of Virology, Faculty of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia.
National Research Center "Kurchatov Institute", Moscow 123182, Russia.
Plants (Basel). 2025 May 26;14(11):1619. doi: 10.3390/plants14111619.
Phlox are ornamentals of great decorative value, grown throughout the world for their attractive flowers. Phlox cultivar collections at the Tsitsin Main Botanical Garden and the Botanical Garden of Lomonosov Moscow State University (both Moscow, Russia) were surveyed for virus diseases. Tobacco streak ilarvirus (TSV), beet ringspot nepovirus (BRSV), and BRSV satellite RNA (satRNA) were first detected in phlox when viromes of symptomatic plants were studied using high-throughput sequencing. The nearly complete genomes of three TSV and BRSV isolates and two BRSV satRNAs were assembled and characterized. TSV isolates shared 96.9-99.7% nucleotide sequence identity and were 82.2-89.1% identical to their closest relatives from broad bean, dahlia, and echinacea. BRSV isolates were distantly related to each other (83.7-89.3% identity) and were closest to those from oxalis and potato. BRSV satRNAs shared 90.6% identity and were 87.8-94.1% identical to satRNAs associated with tomato black ring virus L and S serotypes. Thus, TSV, BRSV, and BRSV satRNA were for the first time detected in a new natural host in Russia, adding to the list of known phlox viruses and expanding information on the host range, geographic distribution, and genetic diversity of these viruses.
福禄考是极具观赏价值的花卉，因其迷人的花朵而在世界各地广泛种植。对俄罗斯莫斯科季米里亚泽夫主植物园和莫斯科国立大学罗蒙诺索夫植物园（均位于莫斯科）的福禄考品种收集物进行了病毒病调查。当使用高通量测序研究有症状植物的病毒组时，首次在福禄考中检测到烟草条纹等轴不稳环斑病毒（TSV）、甜菜环斑线虫传多面体病毒（BRSV）和BRSV卫星RNA（satRNA）。组装并鉴定了三个TSV分离株、三个BRSV分离株的近乎完整的基因组以及两个BRSV satRNA。TSV分离株的核苷酸序列同一性为96.9 - 99.7%，与蚕豆、大丽花和紫锥菊中亲缘关系最近的病毒同一性为82.2 - 89.1%。BRSV分离株彼此亲缘关系较远（同一性为83.7 - 89.3%），与酢浆草和马铃薯中的病毒亲缘关系最近。BRSV satRNA的同一性为90.6%，与番茄黑环病毒L和S血清型相关的satRNA同一性为87.8 - 94.1%。因此，TSV、BRSV和BRSV satRNA首次在俄罗斯的一种新天然寄主中被检测到，增加了已知的福禄考病毒种类，并扩展了关于这些病毒寄主范围、地理分布和遗传多样性的信息。
