The growth of young roots is crucial for the development and yield of rice. However, the molecular mechanisms underlying young rice root development remain unclear. Our research indicates that the rice B-type cytokinin response regulator factor negatively regulates the development of young rice roots. is highly expressed in the root meristematic zone of young rice roots. In transgenic lines overexpressing , the lengths of primary roots and adventitious roots, as well as the corresponding root meristematic zone lengths, are significantly reduced. This is due to a decrease in both the number and size of longitudinal cells in the root meristematic zone. On the one hand, can inhibit root apical cell division and reduce the number of longitudinal cells in the root meristematic zone by affecting the auxin synthesis and transport pathways. On the other hand, ORR3 may directly activate the transcription of cell wall metabolism-related genes, thereby restricting the size of cells in the root meristematic zone. In summary, negatively regulates rice young root growth by responding to cytokinin signals to influence auxin signal transduction and cell wall metabolism pathways, thereby negatively regulating the number and size of cells in the root meristematic zone.