Bawane Pradip, Yele Santosh
Shobhaben Pratapbhai Patel School of Pharmacy & Technology Management, SVKM's NMIMS, Mumbai, 400056, India.
Shri Vile Parle Kelavani Mandal's Institute of Pharmacy, Dhule, Maharashtra, 424001, India.
Int Microbiol. 2025 Jun 19. doi: 10.1007/s10123-025-00682-3.
Constant research is being conducted to discover potent antibacterial and anticancer agents from natural sources like plants and microorganisms. Gram-positive Paenibacillus dendritiformis is a spore-forming bacterium with recognized industrial and economic significance in the production of important biomolecules applicable to the agricultural, pharmaceutical, and biotechnological industries.
The objective of this study was to investigate the antibacterial and cytotoxic potential of bioactives produced by P. dendritiformis isolated from Lonar Lake sediment.
P. dendritiformis was isolated from Lonar Soda Lake and characterized using colony morphology, biochemical, and phylogenetic evaluations. The process was carried out using starch casein broth (SCB), and the bioactives were characterized by LC-MS/MS. The antibacterial assay was performed by the disc diffusion assay. The cytotoxic activity was performed by employing HeLa cell culture. To understand the molecular mechanism, oxidative stress markers, pro-inflammatory markers, and apoptotic markers were evaluated.
LC-MS/MS spectra reveal the presence of phytyl palmitate and phytol acetate. These bioactives from P. dendritiformis exhibit moderately significant to significant antibacterial and cytotoxic activity by substantially inhibiting the HeLa cell viability via MTT assay.
The study demonstrates the antibacterial and cytotoxic potential of bioactive compounds from P. dendritiformis with significantly lower IC values as compared to standard probes.
人们一直在不断开展研究，以从植物和微生物等天然来源中发现强效抗菌和抗癌剂。革兰氏阳性的树状芽孢杆菌是一种形成芽孢的细菌，在生产适用于农业、制药和生物技术产业的重要生物分子方面具有公认的工业和经济意义。
本研究的目的是调查从洛纳湖沉积物中分离出的树状芽孢杆菌所产生的生物活性物质的抗菌和细胞毒性潜力。
从洛纳苏打湖分离出树状芽孢杆菌，并通过菌落形态、生化和系统发育评估对其进行表征。该过程使用淀粉酪蛋白肉汤（SCB）进行，生物活性物质通过液相色谱-串联质谱（LC-MS/MS）进行表征。抗菌试验通过纸片扩散法进行。细胞毒性活性通过使用HeLa细胞培养来进行。为了解分子机制，对氧化应激标志物、促炎标志物和凋亡标志物进行了评估。
LC-MS/MS光谱显示存在植基棕榈酸酯和植醇乙酸酯。这些来自树状芽孢杆菌的生物活性物质通过MTT试验显著抑制HeLa细胞活力，表现出中度显著到显著的抗菌和细胞毒性活性。
该研究证明了树状芽孢杆菌生物活性化合物的抗菌和细胞毒性潜力[注：此处英文原文有误，正确应该是“与标准探针相比，其IC值显著更低”] 。
