Boerman Nicholas A, Robbins Matthew D, Chandra Ambika, Brentano Samantha, Brentano Tom, Bushman B Shaun
Livestock, Forages, and Pasture Management Research Unit, USDA-ARS, 2000 18th St., Woodward, OK 73801, USA.
Forage and Range Research Laboratory, USDA-ARS, 695 North 1100 East, Logan, UT 84322, USA.
G3 (Bethesda). 2025 Aug 6;15(8). doi: 10.1093/g3journal/jkaf133.
Kentucky bluegrass is an important cool-season turfgrass species. However, heat and drought tolerance is an issue. Interspecific hybridization with a related species, Texas bluegrass, is an approach used to improve heat and drought tolerance. We report herein a contig assembly and annotation of Texas bluegrass that was completed and used to measure the population structure of Texas bluegrass, interspecific lines between Texas and Kentucky bluegrass, and the percent allele sharing between advanced interspecific lines and cultivars to Texas bluegrass. The contig assembly was comprised of 367 contigs and spanned 6.6 Gb with 198,746 predicted gene models and an assembly and transcriptome completeness of over 97% as indicated by BUSCO orthologous gene alignment. It was used to call 14,504 high-quality SNPs. A principal component analysis showed separation of populations across the first 3 PCs, explaining 21.5, 11.1, and 5.4%, respectively, of the variation across the populations. Advanced interspecific lines and cultivars diverged from Texas bluegrass while sharing 62-74% of their alleles with Texas bluegrass. Interspecific Texas × Kentucky bluegrasses could be important for improving heat and drought tolerance among bluegrasses.
草地早熟禾是一种重要的冷季型草坪草种。然而，其耐热和耐旱性是个问题。与相关物种德州蓝草进行种间杂交是一种用于提高耐热和耐旱性的方法。我们在此报告了德州蓝草的重叠群组装和注释情况，该工作已完成，并用于测定德州蓝草的群体结构、德州蓝草与草地早熟禾之间的种间品系，以及高级种间品系和品种与德州蓝草之间的等位基因共享百分比。重叠群组装由367个重叠群组成，跨度为6.6 Gb，有198,746个预测基因模型，如BUSCO直系同源基因比对所示，组装和转录组完整性超过97%。它被用于鉴定14,504个高质量单核苷酸多态性（SNP）。主成分分析显示，在前三个主成分上群体出现分离，分别解释了群体间变异的21.5%、11.1%和5.4%。高级种间品系和品种与德州蓝草有所分化，同时与德州蓝草共享62 - 74%的等位基因。德州蓝草与草地早熟禾的种间杂交种对于提高蓝草的耐热和耐旱性可能很重要。
