McAlister R O, Gordon D M
J Parasitol. 1977 Jun;63(3):448-54.
Studies were performed to evaluate several methods for the artificial removal of Plasmodium berghei merozoites from infected mouse erythrocytes. These methods, many of which have been reported to yield free parasites capable of establishing a patent infection when injected into a suitable host, included NH4C1-mediated lysis, complement-mediated immune lysis, pressure filtration, and multiple-burst and continuous-flow sonication. Free parasites isolated from infected mouse blood were examined in vitro under conditions known to support merozoite invasion, and were found to be noninvasive, irrespective of the method used for their isolation. Although all methods tested achieved high degrees of lysis, none removed all intact parasitized erythrocytes. Using multiple-burst and continuous-flow sonication, the infective potential of free parasite preparations could be accounted for solely on the basis of the intact parasitized cells contaminating the free parasite preparations.
开展了多项研究，以评估从受感染小鼠红细胞中人工去除伯氏疟原虫裂殖子的几种方法。这些方法包括氯化铵介导的裂解、补体介导的免疫裂解、压力过滤以及多次脉冲和连续流超声处理，其中许多方法据报道可产生游离寄生虫，将其注入合适宿主后能够引发显性感染。从受感染小鼠血液中分离出的游离寄生虫在已知支持裂殖子入侵的条件下进行体外检测，结果发现无论采用何种分离方法，这些寄生虫均无侵袭能力。尽管所有测试方法均实现了高度裂解，但没有一种方法能去除所有完整的被寄生红细胞。使用多次脉冲和连续流超声处理时，游离寄生虫制剂的感染潜力完全可归因于污染游离寄生虫制剂的完整被寄生细胞。
