Jiang Huan-Ying, Zhang Dong-Xu, Sun Zhong-Ren, Jiao Xue-Feng, Zhou Ming-Yuan, Huang Fei, Luo Zi-Ru, Sun Mei-Qi, Kai Xin, Yin Hong-Na
Heilongjiang University of Chinese Medicine, Harbin 150040, China.
Department of Acupuncture and Moxibustion, The Second Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin 150000.
Zhen Ci Yan Jiu. 2025 Jun 25;50(6):633-640. doi: 10.13702/j.1000-0607.20240070.
OBJECTIVES: To observe the effect of electroacupuncture (EA) at "Jiaji" (EX-B2) on inhibiting neuronal apoptosis in rats with spinal cord injury based on the Janus tyrosine kinase (JAK)/signal transducer and activator of transcription (STAT) signaling pathway, so as to explore the underlying mechanism of EA in the treatment of spinal cord injury. METHODS: A total of 36 SD rats were randomly divided into sham operation group, spinal cord injury group, and Jiaji EA group, with 12 rats in each group. The spinal cord injury model was established using Allen's method. The Jiaji EA group was treated with EA at EX-B2 (once a day, 30 min each time, for a total of 14 d). After the treatment, the Basso Beattie Bresnahan (BBB) score was used to evaluate the hindlimb motor function of rats in each group. HE staining was used to observe the morphological changes of spinal cord tissues. TUNEL staining was used to observe the pathological changes and neuronal apoptosis rate of the spinal cord. Western blot was used to detect the relative expression levels of phosphorylated (p)-JAK2, p-STAT3, B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), and Caspase-3 proteins in the spinal cord. RESULTS: Compared with the sham operation group at the same time point, the BBB score decreased (<0.01), the spinal cord tissue structure in the injury area was disordered, the cell body shrank, and the neuronal apoptosis rate increased (<0.01), the expression levels of p-JAK2, p-STAT3, Bax, and caspase-3 proteins increased (<0.01) while the expression level of Bcl-2 protein decreased (<0.01) in rats of the spinal cord injury group. Compared with the spinal cord injury group, the BBB score increased on the 7 and 14 day of intervention (<0.01), the spinal cord tissue structure tended to be normal, the degree of neuronal damage was reduced, and the apoptosis rate decreased (<0.01), the expression levels of p-JAK2, p-STAT3, Bax, and caspase-3 proteins decreased (<0.01, <0.05), while the expression level of Bcl-2 protein increased (<0.05) in rats of the Jiaji EA group. CONCLUSIONS: EA at EX-B2 can improve the motor function of rats with spinal cord injury, up-regulate the expressions of anti-apoptotic factors, down-regulate the expressions of pro-apoptotic factors, and reduce neuronal apoptosis in spinal cord injury. The mechanism may be related to the inhibition of the activation of the JAK2/STAT3 signaling pathway.
目的：基于Janus酪氨酸激酶（JAK）/信号转导及转录激活因子（STAT）信号通路，观察电针“夹脊”（EX - B2）对脊髓损伤大鼠神经元凋亡的抑制作用，以探讨电针治疗脊髓损伤的潜在机制。 方法：将36只SD大鼠随机分为假手术组、脊髓损伤组和夹脊电针组，每组12只。采用Allen法建立脊髓损伤模型。夹脊电针组于EX - B2处进行电针治疗（每日1次，每次30分钟，共14天）。治疗后，采用Basso Beattie Bresnahan（BBB）评分评估各组大鼠后肢运动功能。苏木精-伊红（HE）染色观察脊髓组织形态学变化。TUNEL染色观察脊髓病理变化及神经元凋亡率。蛋白质免疫印迹法检测脊髓中磷酸化（p）-JAK2、p-STAT3、B细胞淋巴瘤-2（Bcl-2）、Bcl-2相关X蛋白（Bax）和半胱天冬酶-3（Caspase-3）蛋白的相对表达水平。 结果：与同一时间点的假手术组相比，脊髓损伤组大鼠BBB评分降低（<0.01），损伤区域脊髓组织结构紊乱，细胞体萎缩，神经元凋亡率增加（<0.01），p-JAK2、p-STAT3、Bax和Caspase-3蛋白表达水平升高（<0.01），而Bcl-2蛋白表达水平降低（<0.01）。与脊髓损伤组相比，夹脊电针组大鼠在干预第7天和第14天BBB评分升高（<0.01），脊髓组织结构趋于正常，神经元损伤程度减轻，凋亡率降低（<0.01），p-JAK2、p-STAT3、Bax和Caspase-3蛋白表达水平降低（<0.01，<0.05），而Bcl-2蛋白表达水平升高（<0.05）。 结论：EX - B2处电针可改善脊髓损伤大鼠的运动功能，上调抗凋亡因子表达，下调促凋亡因子表达，减少脊髓损伤中的神经元凋亡。其机制可能与抑制JAK2/STAT3信号通路的激活有关。
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2025-6-15
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