Pan Juan, Zhang Jia, Hu Panjie, Yao Zhuocheng, Zhang Xiaotuan, Zhou Tieli, Shen Mo
Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, Wenzhou, Zhejiang, 325000, People's Republic of China.
School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, People's Republic of China.
Int J Nanomedicine. 2025 Jun 19;20:7811-7827. doi: 10.2147/IJN.S515798. eCollection 2025.
Bacterial resistance to carbapenems is on the rise, and the failure of common antimicrobials poses a serious challenge in treating infections caused by drug-resistant strains. In this scenario, nanomaterials have received widespread attention in medicine.
In this study, the activity of daidzein-decorated gold nanoparticles (Daidzein_Au NPs) against carbapenem-resistant Enterobacteriaceae (CRE) strains was validated via in vivo and in vitro experiments, such as the microdilution broth method, growth curves, time-killing assays, and an abdominal infection model in mice. The potential mechanisms involved were examined using fluorescence quantification of N-phenyl-1-naphthylamine, propidium iodide, and reactive oxygen species (ROS) levels and transcriptomic analysis.
Face-centered cubic Daidzein_Au NPs with an average size of 25.78 nm and a negative surface charge were successfully synthesized. The minimum inhibitory concentration (MIC) of Daidzein_Au NPs against the tested CRE strains was 8-16 μg/mL, and they exerted a good antimicrobial effect during the dynamic killing process. In vivo experiments showed that Daidzein_Au NPs can significantly enhance the survival rate (100%) and reduce the colony load of ascites in mice ( < 0.05). Furthermore, Daidzein_Au NPs destroyed the permeability of bacterial cell membranes, increased the production of ROS ( < 0.05), and affected the metabolism of CRE strains to play a role in killing.
Daidzein_Au NPs exhibit excellent antibacterial activity and are expected to become a promising solution to the threats posed by CRE strains.
细菌对碳青霉烯类药物的耐药性正在上升，常见抗菌药物的失效给治疗耐药菌株引起的感染带来了严峻挑战。在这种情况下，纳米材料在医学领域受到了广泛关注。
在本研究中，通过体内和体外实验，如微量肉汤稀释法、生长曲线、时间杀菌试验以及小鼠腹部感染模型，验证了大豆苷元修饰的金纳米颗粒（Daidzein_Au NPs）对耐碳青霉烯类肠杆菌科（CRE）菌株的活性。使用N-苯基-1-萘胺、碘化丙啶和活性氧（ROS）水平的荧光定量以及转录组分析来研究其中涉及的潜在机制。
成功合成了平均粒径为25.78 nm且表面带负电荷的面心立方Daidzein_Au NPs。Daidzein_Au NPs对测试的CRE菌株的最低抑菌浓度（MIC）为8 - 16μg/mL，并且在动态杀菌过程中发挥了良好的抗菌作用。体内实验表明，Daidzein_Au NPs可显著提高小鼠的存活率（100%）并降低小鼠腹水中的菌落载量（<0.05）。此外，Daidzein_Au NPs破坏了细菌细胞膜的通透性，增加了ROS的产生（<0.05），并影响了CRE菌株的代谢以发挥杀菌作用。
Daidzein_Au NPs表现出优异的抗菌活性，有望成为应对CRE菌株所带来威胁的一种有前景的解决方案。
Zotero 插件
