Tong Sen-Miao, Qi Dan-Yi, Tang Xin-Yue, Shao Yan-Zhi, Hu Shi-Tong, Zheng Yu-Piao, Zheng Xin-Yun, Yan Ling-Li, Feng Ming-Guang
College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, China.
Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
Insects. 2025 May 27;16(6):565. doi: 10.3390/insects16060565.
The high costs of mycoinsecticides restrain their extensive application in green agriculture. Two six-week field trials were performed in spring to test synergistic effects of fungal-chemical interactions against faba bean aphid () populations in Zhejiang, China. The treatments (three 50-m plots each) in each trial included ZJU435 (Bb) and imidacloprid (ImD) applied biweekly at recommended rates (1.5 × 10 conidia and 45 a.i. g ha) and co-applied at reduced rates of 2/3Bb, 1/2Bb, and 1/3Bb plus 1/5ImD, which reduced the cost of the mycoinsecticide by 32-65%. During the first three weeks at 1.3-12.5 °C (daily means), the aphid population steadily increased to a peak in the blank control, and it was much more effectively suppressed by ImD than by Bb and Bb + ImD treatments. As the weather subsequently warmed to 8.7-21.0 °C, the aphid population was increasingly suppressed by the Bb and Bb + ImD treatments, which showed suppressive effects superior or close to those of ImD on days 35 and 42. Percent efficacy values ( = 36) of ImD, 2/3Bb + 1/5ImD, 1/2Bb + 1/5ImD, Bb, and 1/3Bb + 1/5ImD in two six-week trials averaged respectively 86%, 66%, 62.2%, 59.4%, and 58.4%, which significantly differed from one another ( < 0.01). Conclusively, low-rate co-applications of fungal/chemical insecticides offer a 'low-cost-high-efficacy' strategy to promote extensive mycoinsecticide application for sustainable aphid control.
杀真菌昆虫剂的高成本限制了它们在绿色农业中的广泛应用。春季进行了两项为期六周的田间试验，以测试真菌 - 化学相互作用对中国浙江蚕豆蚜（）种群的协同效应。每个试验中的处理（每个处理三个50米的地块）包括以推荐剂量（1.5×10分生孢子和45克活性成分/公顷）每两周施用一次的ZJU435（球孢白僵菌）和吡虫啉（ImD），以及以2/3Bb、1/2Bb和1/3Bb加1/5ImD的降低剂量共同施用，这将杀真菌昆虫剂的成本降低了32 - 65%。在1.3 - 12.5°C（日均温度）的前三周，空白对照中的蚜虫种群稳步增加至峰值，吡虫啉对其抑制效果比球孢白僵菌和球孢白僵菌 + 吡虫啉处理更有效。随着天气随后升温至8.7 - 21.0°C，球孢白僵菌和球孢白僵菌 + 吡虫啉处理对蚜虫种群的抑制作用越来越强，在第35天和第42天显示出优于或接近吡虫啉的抑制效果。在两项为期六周的试验中，吡虫啉、2/3Bb + 1/5ImD、1/2Bb + 1/5ImD、球孢白僵菌和1/3Bb + 1/5ImD的防效值（ = 36）平均分别为86%、66%、62.2%、59.4%和58.4%，它们之间存在显著差异（ < 0.01）。总之，低剂量真菌/化学杀虫剂共同施用提供了一种“低成本高效能”策略，以促进杀真菌昆虫剂广泛应用于可持续蚜虫防治。
