Koizumi S, Sone T, Kimura M
J Cell Physiol. 1985 Nov;125(2):223-8. doi: 10.1002/jcp.1041250208.
Metallothionein (MT) synthesis in rabbit kidney-derived RK-13 cells was studied. In response to Cd2+, RK-13 cells synthesized proteins closely similar in chromatographic and electrophoretic behaviors to the liver MTs induced in Cd2+-injected rabbit. These proteins were specifically immunoprecipitated by anti-mouse liver MT-II serum. The rate of RK-13 thionein (apoprotein of MT) synthesis rapidly increased after exposure to 1 microgram/ml of Cd2+, and reached the maximum in 7 h. The dose-response curve for the synthesis was biphasic; a sharp increase up to 0.5 microgram/ml and a slower increase at higher concentrations. RK-13 cells retained kidney-specific properties in terms of responsiveness of thionein synthesis to inducers; The MTs were inducible also by Zn2+ and probably by Hg2+, but not by dexamethasone. This system would therefore be a useful model in vitro for studying the regulation of MT synthesis in kidney cells.
对兔肾来源的RK - 13细胞中金属硫蛋白（MT）的合成进行了研究。响应Cd2 +，RK - 13细胞合成的蛋白质在色谱和电泳行为上与注射Cd2 +的兔肝脏中诱导产生的MT非常相似。这些蛋白质被抗小鼠肝脏MT - II血清特异性免疫沉淀。暴露于1微克/毫升的Cd2 +后，RK - 13硫蛋白（MT的脱辅基蛋白）的合成速率迅速增加，并在7小时内达到最大值。合成的剂量反应曲线呈双相；在0.5微克/毫升时急剧增加，在较高浓度时增加较慢。就硫蛋白合成对诱导剂的反应性而言，RK - 13细胞保留了肾脏特异性特性；MT也可被Zn2 +诱导，可能也可被Hg2 +诱导，但不能被地塞米松诱导。因此，该系统将是体外研究肾细胞中MT合成调控的有用模型。
