Cheng Ying, Zhou Yuhang, Li Cao
Guizhou Institute of Plant Protection, Guiyang 550006, China.
Guizhou Key Laboratory of Agricultural Biosecurity, Guiyang 550006, China.
Insects. 2025 Jun 19;16(6):643. doi: 10.3390/insects16060643.
The effects of the gene encoding the ecdysone receptor () on the reproduction of the ladybug was evaluated. transcription was measured by quantitative real-time PCR in ladybug adults reared on artificial diets with and without 20-hydroxyecdysone (20E). expression levels in 5 d old male and female ladybugs supplied with the 20E-amended artificial diet were lower than expression levels in ladybugs reared on an artificial diet lacking 20E. However, expression levels in 10 d old ladybugs supplied with the 20E artificial diet were higher than those lacking 20E supplementation. The regulatory effects of were studied in female and male ladybugs by RNA interference. expression in female ladybugs injected with -dsRNA was significantly downregulated after 5 d but remained unaffected in 10 d old females. expression levels in males microinjected with -dsRNA were significantly lower at 5 and 10 d after microinjection than -dsRNA-treated males. The ovary volume in females injected with -dsRNA at 5 d was smaller than females microinjected with -dsRNA, but volumes at 10 d were larger than in -dsRNA-treated females. The testes of males injected with -dsRNA were larger than those injected with -dsRNA at 5 d but the testes at 10 d after injection with -dsRNA were smaller than those injected with -dsRNA. When females were microinjected with -dsRNA and mated with noninjected males, egg production decreased by 34.80% for 20 days. When males were microinjected with -dsRNA and mated with noninjected females, egg production decreased by 30.38% for 20 days. Injection of female and male ladybugs with -dsRNA had no significant effect on egg hatching rates. Our results show that plays an important role in the development of reproductive organs and egg development in .
评估了编码蜕皮激素受体（）的基因对瓢虫繁殖的影响。通过定量实时PCR在添加和不添加20-羟基蜕皮激素（20E）的人工饲料饲养的瓢虫成虫中测量转录水平。在提供20E改良人工饲料的5日龄雄性和雌性瓢虫中，表达水平低于在缺乏20E的人工饲料饲养的瓢虫中的表达水平。然而，在提供20E人工饲料的10日龄瓢虫中，表达水平高于未添加20E的瓢虫。通过RNA干扰研究了在雌性和雄性瓢虫中的调控作用。注射-dsRNA的雌性瓢虫在5天后表达明显下调，但在10日龄雌性中未受影响。显微注射-dsRNA的雄性在注射后5天和10天的表达水平明显低于注射-dsRNA的雄性。5天时注射-dsRNA的雌性卵巢体积小于显微注射-dsRNA的雌性，但10天时的体积大于注射-dsRNA处理的雌性。注射-dsRNA的雄性睾丸在5天时大于注射-dsRNA的睾丸，但注射-dsRNA后10天的睾丸小于注射-dsRNA的睾丸。当雌性显微注射-dsRNA并与未注射的雄性交配时，20天内产卵量下降了34.80%。当雄性显微注射-dsRNA并与未注射的雌性交配时，20天内产卵量下降了30.38%。向雌性和雄性瓢虫注射-dsRNA对卵孵化率没有显著影响。我们的结果表明，在瓢虫生殖器官发育和卵发育中起重要作用。
