Badlaeva Alina, Tregubova Anna, Asaturova Aleksandra, Sukhikh Gennady
National Medical Research Center for Obstetrics, Gynecology and Perinatology Named After Academician V.I. Kulakov of the Ministry of Health of Russia, Bldg. 4, Oparina Street, 117513 Moscow, Russia.
Department of Pathological Anatomy and Clinical Pathological Anatomy, Pirogov Russian National Research Medical University, Bldg. 1, Ostrovitianov Street, 117997 Moscow, Russia.
Diagnostics (Basel). 2025 Jun 11;15(12):1479. doi: 10.3390/diagnostics15121479.
: According to recent reports, the mutation in serous borderline tumors (SBTs) plays a protective role against progression to low-grade serous carcinoma through oncogene-induced senescence. One consequence of this is the appearance of eosinophilic cells (ECs). The aim of the current study was to determine the interobserver reproducibility of ECs and their predictive significance for the detection of the mutation in SBTs. : The study was conducted using 63 cases of ovarian SBTs. Three gynecological pathologists, blinded to each tumor's mutation status, assessed the presence of ECs. Immunohistochemical staining with p16 and Ki-67 was performed to validate ECs. Mutational analysis was carried out using targeted NGS. : Genetic analysis revealed 30 -mutated, 1 -mutated, and 9 -mutated SBTs. ECs were identified by the majority of pathologists (two or three) in 78% of the -mutated and 11% of the wild-type tumors with other mutations ( < 0.0001). The interobserver reproducibility of the presence of ECs was substantial (κ = 0.66). ECs validated with p16/Ki-67 were identified in 92.6% of the -mutated and in 13.8% of the wild-type tumors with other mutations ( < 0.0001). For the ECs identified by the majority of pathologists, the sensitivity and specificity when predicting the mutation were 77.8% and 88.9%, respectively. For the ECs validated with p16/Ki-67, the sensitivity and specificity when predicting the mutation were 95.3% and 90.5%, respectively. : Overall, these results suggest that ECs in SBTs have potential association with the mutation.
根据最近的报道，浆液性交界性肿瘤（SBTs）中的突变通过癌基因诱导的衰老对进展为低级别浆液性癌起到保护作用。其结果之一是嗜酸性细胞（ECs）的出现。本研究的目的是确定ECs在观察者间的可重复性及其对SBTs中突变检测的预测意义。
该研究使用了63例卵巢SBTs病例。三位妇科病理学家在不知道每个肿瘤突变状态的情况下评估ECs的存在情况。采用p16和Ki-67免疫组化染色来验证ECs。使用靶向二代测序进行突变分析。
基因分析显示30例有突变、1例有突变和9例有其他突变的SBTs。大多数病理学家（两名或三名）在78%的有突变肿瘤和11%的有其他突变的野生型肿瘤中识别出了ECs（P<0.0001）。ECs存在情况在观察者间的可重复性很高（κ=0.66）。在92.6%的有突变肿瘤和13.8%的有其他突变的野生型肿瘤中识别出了经p16/Ki-67验证的ECs（P<0.0001）。对于大多数病理学家识别出的ECs，预测突变时的敏感性和特异性分别为77.8%和88.9%。对于经p16/Ki-67验证的ECs，预测突变时的敏感性和特异性分别为95.3%和90.5%。
总体而言，这些结果表明SBTs中的ECs与突变可能存在关联。
