Guo Shijiao, Wang Kaiming, Zhang Hua, Luo Chaochao, Zhao Zixuan, Tong Jinjin
Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China.
College of Veterinary Medicine, Beijing University of Agriculture, Beijing 102206, China.
Microorganisms. 2025 May 23;13(6):1192. doi: 10.3390/microorganisms13061192.
(GBS) is a major pathogen causing mastitis in dairy cows while causing oxidative stress. Matrine is an alkaloid compound extracted from the roots of , a plant used in traditional Chinese medicine. It possesses antioxidant, immunomodulatory, anti-inflammatory, and pro-apoptotic properties. The aim of this study was to investigate the regulatory effects of matrine on the virulence of the ATCC strain (ATCC13813) and clinical GBS strains by transcriptome analysis and qRT-PCR validation. The results showed that the ABC transporter, peptidoglycan biosynthesis, and quorum-sensing pathways were significantly altered in ATCC (4 mg/mL) and GBS (12 mg/mL) strains after matrine treatment at MIC concentrations. Additionally, genes related to invasion and immune escape, including , , , and , and genes related to the expression of adhesion and virulence factors, such as , , , and , were significantly downregulated ( < 0.05). Overall, these data suggest that matrine effectively inhibits the virulence genes of GBS, thereby reducing immune evasion and infection by decreasing the synthesis of capsular polysaccharides and host cell adhesion.
无乳链球菌（GBS）是引起奶牛乳腺炎的主要病原体，同时会引发氧化应激。苦参碱是从一种传统中药植物的根部提取的生物碱化合物。它具有抗氧化、免疫调节、抗炎和促凋亡特性。本研究旨在通过转录组分析和qRT-PCR验证来研究苦参碱对ATCC菌株（ATCC13813）和临床GBS菌株毒力的调节作用。结果表明，在MIC浓度的苦参碱处理后，ATCC（4 mg/mL）和GBS（12 mg/mL）菌株中的ABC转运蛋白、肽聚糖生物合成和群体感应途径发生了显著改变。此外，与侵袭和免疫逃逸相关的基因，包括[此处原文缺失具体基因名称]，以及与黏附及毒力因子表达相关的基因，如[此处原文缺失具体基因名称]，均显著下调（P < 0.05）。总体而言，这些数据表明苦参碱可有效抑制GBS的毒力基因，从而通过减少荚膜多糖的合成和宿主细胞黏附来降低免疫逃逸和感染。
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