Emerging tick-borne infections pose growing public health threats, causing global disease burdens and economic losses. In this study, tick-borne pathogens were detected in ticks collected from ruminants in 19 sites of Khyber Pakhtunkhwa Province, Pakistan, between 2023 and 2024. A total of 989 ticks, belonging to five species, i.e., , , , , and , were tested by specific PCR followed by Sanger sequencing. In total, fourteen pathogens including two species, three species, three species, one species, and five species were identified, with an overall infection rate of 20.2% (95% CI: 17.7-22.7%). Phylogenetic analyses revealed two undefined species: Ehrlichia hyalommae was exclusively detected in . ticks, while Ehrlichia rhipicephalis was only found in . Additionally, an undefined , provisionally named Rickettsia pakistanensis, was identified, which is phylogenetically close to in North Asia and in Africa, suggesting its potential pathogenicity to humans. Although coinfections of two pathogens were observed, the coinfection rates were quite low. The findings revealed a significant diversity of tick-borne pathogens in Pakistani ticks, which may pose risks to livestock and humans.