Martínez-Pérez Mireya, Velandia-Álvarez Sebastian, Vidal-Verdú Cristina, Álvarez-Rodríguez Beatriz, Geller Ron
Institute for Integrative Systems Biology (I2SysBio), Universitat de València-Consejo Superior de Investigaciones Científicas (UV-CSIC), 46980 Valencia, Spain.
Viruses. 2025 May 30;17(6):796. doi: 10.3390/v17060796.
Enteroviruses are a group of highly prevalent human pathogens responsible for a wide range of illnesses, ranging from common cold symptoms to life-threatening diseases. A deep understanding of enterovirus biology, evolution, and host interaction is required for the development of effective vaccines and antivirals. Recombinant reporter viruses encoding luminescent or fluorescent proteins have been developed to facilitate such investigation. In this work, using coxsackievirus B3 as our model, we analyze how the insertion of fluorescent reporter genes at three distinct sites within the viral polyprotein affects viral fitness, identifying the most tolerant site for reporter insertion. We then describe a set of experimental workflows for measuring viral fitness, sera neutralization, antiviral efficacy, and recombination using fluorescent reporter viruses. The high homology between different enteroviruses suggests these assays can be readily adapted to study additional members of this medically and economically relevant group of viruses.
肠道病毒是一类高度流行的人类病原体，可导致从普通感冒症状到危及生命的疾病等广泛疾病。开发有效的疫苗和抗病毒药物需要深入了解肠道病毒的生物学、进化以及与宿主的相互作用。已开发出编码发光或荧光蛋白的重组报告病毒以促进此类研究。在这项工作中，我们以柯萨奇病毒B3为模型，分析在病毒多聚蛋白内三个不同位点插入荧光报告基因如何影响病毒适应性，确定报告基因插入的最耐受位点。然后，我们描述了一套使用荧光报告病毒测量病毒适应性、血清中和、抗病毒效力和重组的实验工作流程。不同肠道病毒之间的高度同源性表明，这些检测方法可轻松适用于研究这一在医学和经济上具有重要意义的病毒组中的其他成员。
