Short-Season Direct-Seeded Cotton Cultivation Under Once-Only Irrigation Throughout the Growing Season: Investigating the Effects of Planting Density and Nitrogen Application.

To identify optimal strategies for high-yield and high-efficiency cultivation under a "short-season direct-seeded cotton with once-only irrigation" regime, we conducted two-year field experiments (2022 and 2023) using a split-plot factorial design with three planting densities (30,000 (D1), 45,000 (D2), and 60,000 (D3) plants·ha) and three nitrogen application rates (150 (N1), 180 (N2), and 210 (N3) kg·ha). Our study systematically examined how these treatment combinations influenced canopy architecture, physiological traits, yield components, and fiber quality. The results showed that increased planting density significantly enhanced plant height, the leaf area index (LAI), and the number of fruiting branches, with the highest density (D3) contributing to a more compact and efficient canopy. Moderate nitrogen input (N2) significantly increased peroxidase (POD) activity, reduced malondialdehyde (MDA) accumulation, delayed functional leaf senescence, and prolonged the canopy's photosynthetic performance. A significant interaction between planting density and nitrogen application was observed. The D3N2 treatment (high density with moderate nitrogen) consistently achieved the highest fruiting branch count, boll number per plant, and yields of both seed cotton and lint in both years, while maintaining stable fiber quality. This indicates its strong capacity to balance high yield with quality and maintain physiological resilience. By contrast, the D1N1 treatment (low density and low nitrogen) exhibited a loose canopy, premature photosynthetic decline, and the lowest yield. The D3N3 treatment (high density and high nitrogen) promoted vigorous early growth but reduced stress tolerance during later growth stages, leading to yield instability. These findings demonstrate that moderately increasing planting density while maintaining appropriate nitrogen levels can effectively optimize canopy structure, improve stress resilience, and enhance yield under short-season direct-seeded cotton systems with once-only irrigation. This provides both theoretical underpinning and practical guidance for achieving stable and efficient cotton production under such systems.