Fletcher K A, Canning M V, Theakston R D
Ann Trop Med Parasitol. 1977 Jun;71(2):125-30. doi: 10.1080/00034983.1977.11687170.
Electrophoresis of red-cell extracts from control and malaria-infected animals on 'Cellogel' demonstrated the absence of G-6-PD activity of 'parasite' origin, although 6-PGD activity was present. An identical 6-PGD isoenzyme was found in mice, rats and hamsters infected with the same strain of Plasmodium berghei indicating the parasite as the source of the enzyme. A similar 6-PGD isoenzyme was also found in a few preliminary experiments with P. knowlesi-infected monkey erythrocytes. The implications of these findings are discussed in relation to previous studies and also to the status of the pentose phosphate pathway in plasmodial metabolism.
对来自对照动物和感染疟疾动物的红细胞提取物在“Cellogel”上进行电泳分析，结果显示虽然存在6 -磷酸葡萄糖脱氢酶（6 - PGD）活性，但不存在源自“寄生虫”的葡萄糖-6-磷酸脱氢酶（G - 6 - PD）活性。在感染同一伯氏疟原虫株的小鼠、大鼠和仓鼠中发现了相同的6 - PGD同工酶，表明该酶来源于寄生虫。在一些用诺氏疟原虫感染的猴红细胞进行的初步实验中也发现了类似的6 - PGD同工酶。结合先前的研究以及磷酸戊糖途径在疟原虫代谢中的地位，对这些发现的意义进行了讨论。
