Kamounah Sarah, Sarathi Arjun, Elisabeth Sørensen Christiane, Arumugam Manimozhiyan, Pedersen Anne Marie Lynge
Section for Oral Biology and Immunopathology/Oral Medicine & Pathology, Department of Odontology, Faculty of Health and Medical Sciences, University of Copenhagen, Nørre Allé 20, 2200 Copenhagen N, Denmark.
Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen N, Denmark.
J Microbiol. 2025 Jun;63(6):e2501030. doi: 10.71150/jm.2501030. Epub 2025 Jun 30.
This study aimed to determine if the microbiota in four different oral sites and the oral health status differ between patients with primary Sjögren's syndrome (pSS), non-pSS sicca symptoms, and healthy controls. All participants underwent an interview and clinical oral examination. Stimulated whole saliva (SWS), supragingival plaque (SGP), buccal mucosa tissue (BLM), and tongue scrape (TGS) samples from 23 pSS patients, 36 patients with sicca symptoms, not fulfilling the classification criteria for pSS (non-pSS sicca), and 21 age-matched healthy controls (HC) were analyzed using V3-V4 16S rRNA gene amplicon sequencing, and determination of amplicon sequence variants (ASVs). PSS and non-pSS sicca patients did not differ with respect to oral health status, saliva flow rates, abundance of predominant genera, relative abundance on genus level or bacterial diversity in any of the oral sites. Both patient groups differed significantly from the healthy control group in the abundance of 61 ASVs across all sites. The alpha-diversity was lower in SGP from non-pSS sicca patients (p = 0.019), and in TGS from pSS patients (p = 0.04). The proportion of variation in the beta-diversity across all four sites could be explained by the diagnosis (pSS, non-pSS sicca, and HC). However, subgrouping of patients according to their stimulated salivary flow rates (SWS > 0.7 ml/min versus SWS ≤ 0.7 ml/min), revealed significantly different abundance of three ASVs in SWS, 11 in SGP, and six in TGS. Our findings suggest that hyposalivation rather than pSS itself modifies the microbial composition in oral site-specific patterns leading to oral diseases.
本研究旨在确定原发性干燥综合征（pSS）患者、非pSS干燥症状患者和健康对照者在四个不同口腔部位的微生物群以及口腔健康状况是否存在差异。所有参与者均接受了访谈和临床口腔检查。使用V3-V4 16S rRNA基因扩增子测序及扩增子序列变异体（ASV）测定，对23例pSS患者、36例有干燥症状但不符合pSS分类标准的患者（非pSS干燥）以及21名年龄匹配的健康对照者（HC）的刺激性全唾液（SWS）、龈上菌斑（SGP）、颊黏膜组织（BLM）和舌刮拭物（TGS）样本进行了分析。PSS患者和非pSS干燥患者在口腔健康状况、唾液流速、主要菌属丰度、属水平相对丰度或任何口腔部位的细菌多样性方面均无差异。两组患者在所有部位61个ASV的丰度上均与健康对照组存在显著差异。非pSS干燥患者的SGP中α多样性较低（p = 0.019），pSS患者的TGS中α多样性较低（p = 0.04）。所有四个部位β多样性的变异比例可由诊断（pSS、非pSS干燥和HC）解释。然而，根据刺激性唾液流速对患者进行亚组分析（SWS > 0.7 ml/min与SWS≤0.7 ml/min），发现SWS中有3个ASV、SGP中有11个ASV和TGS中有6个ASV的丰度存在显著差异。我们的研究结果表明，唾液分泌减少而非pSS本身以口腔部位特异性模式改变微生物组成，从而导致口腔疾病。
