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针对定植相关基因的免疫球蛋白Y抗体可阻断……的生长和感染。 （原文此处“of”后缺少具体内容）

Immunoglobulin Y antibodies against colonization-related genes block the growth and infection of .

作者信息

Deng Shiyuan, Luo Xiaoling, Du Yunxiao, Elbaiomy Rania G, He Weihan, Guo Rong, El-Sappah Ahmed H, Jian Xiaohong, Xie Yongmei, Bakeer Mohammed, Li Zaixin, Zhang Zhi

机构信息

Department of Biological Engineering, Sichuan University of Science & Engineering, Zigong, China.

Department of Gastroenterology, Fushun People's Hospital, Zigong, China.

出版信息

Front Immunol. 2025 Jun 18;16:1582250. doi: 10.3389/fimmu.2025.1582250. eCollection 2025.

DOI:10.3389/fimmu.2025.1582250
PMID:40607440
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12213714/
Abstract

INTRODUCTION

Immunoglobulin Y (IgY) has emerged as a promising antibody therapy for the eradication of () independent of antibiotics. However, the roles and differences of IgY antibodies targeting various genes against remain unclear.

METHODS

The recombinant antigens of five colonization-related genes - , , , , and - are prepared using a prokaryotic expression system and then subject to immunize laying hens for IgY production. Subsequently, their biological activities are evaluated, including blocking bacterial growth, attenuating infection in GES-1 cells, and eradicating in gastritis mouse models.

RESULTS

These IgY antibodies can recognize the full-length antigens of and exhibit a direct inhibitory effect on the growth and infection of with dose-dependent characteristics. Among these, anti- IgY shows greater antibacterial activity in inhibiting growth and preventing adhesion to GES-1 cells. Oral administration of these IgY antibodies for two weeks (20.0 mg·kg·day) achieves a 25% to 37.5% eradication rate of infection in mice. Interestingly, combination treatment with these IgY antibodies, based on their different roles, enhances antibacterial benefits and significantly promotes the recovery of gastrointestinal function.

CONCLUSION

Our results indicate that IgY antibodies against colonization-related genes can directly block the growth and infection of , and combination treatment with these antibodies offers more advantages in combating .

摘要

引言

免疫球蛋白Y（IgY）已成为一种有前景的抗体疗法，可独立于抗生素根除（）。然而，针对各种基因的IgY抗体对（）的作用和差异仍不清楚。

方法

使用原核表达系统制备五个与定植相关基因——（）、（）、（）、（）和（）的重组抗原，然后用于免疫产蛋母鸡以生产IgY。随后，评估它们的生物学活性，包括阻断细菌生长、减轻在GES-1细胞中的感染以及在胃炎小鼠模型中根除（）。

结果

这些IgY抗体可识别（）的全长抗原，并对（）的生长和感染表现出直接抑制作用，具有剂量依赖性特征。其中，抗（）IgY在抑制（）生长和防止其黏附于GES-1细胞方面表现出更大的抗菌活性。以20.0 mg·kg·天的剂量口服这些IgY抗体两周，可使小鼠体内（）感染的根除率达到25%至37.5%。有趣的是，基于这些IgY抗体的不同作用进行联合治疗，可增强抗菌效果并显著促进胃肠功能的恢复。

结论

我们的结果表明，针对与定植相关基因的IgY抗体可直接阻断（）的生长和感染，并且这些抗体的联合治疗在对抗（）方面具有更多优势。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/12213714/afec88ef54f7/fimmu-16-1582250-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/12213714/7ec326ed7343/fimmu-16-1582250-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/12213714/9d9df2d941d9/fimmu-16-1582250-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/12213714/eb81b8da0afb/fimmu-16-1582250-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/12213714/166faecad581/fimmu-16-1582250-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/12213714/afec88ef54f7/fimmu-16-1582250-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/12213714/7ec326ed7343/fimmu-16-1582250-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/12213714/9d9df2d941d9/fimmu-16-1582250-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/12213714/eb81b8da0afb/fimmu-16-1582250-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/12213714/166faecad581/fimmu-16-1582250-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/12213714/afec88ef54f7/fimmu-16-1582250-g005.jpg

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本文引用的文献

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Reduction of UreB and CagA expression level by siRNA construct in Helicobacter pylori strain SS1.幽门螺杆菌 SS1 株中 siRNA 构建物对 UreB 和 CagA 表达水平的降低作用。
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