De Backer Jonas, De Veirman Lindsy, De Clercq Inge
Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium, Technologiepark 71.
VIB Center for Plant Systems Biology, Ghent, Belgium, Technologiepark 71.
Methods Mol Biol. 2025;2953:167-187. doi: 10.1007/978-1-0716-4694-6_11.
A wide range of technologies have been developed to study protein-protein interactions and to map comprehensive protein interaction networks. In recent years, advances in proximity labeling methods, coupled to protein identification by mass spectrometry, have led to exponential growth in the popularity of these methods. In this chapter, we present a detailed protocol using TurboID in Arabidopsis thaliana seedlings, using membrane-bound transcription factors as a case study. Additionally, we outline the workflow for analyzing mass spectrometry data to identify putative protein interactors.
人们已经开发出了各种各样的技术来研究蛋白质-蛋白质相互作用，并绘制全面的蛋白质相互作用网络。近年来，邻近标记方法的进展，再加上通过质谱法进行蛋白质鉴定，使得这些方法的受欢迎程度呈指数级增长。在本章中，我们以膜结合转录因子为案例研究，展示了在拟南芥幼苗中使用TurboID的详细方案。此外，我们概述了分析质谱数据以鉴定假定蛋白质相互作用体的工作流程。
