Pérez-Gómez Olivia, Rohra-Benítez Sonia, Domínguez-Maqueda Marta, Cerezo Isabel M, Galafat Alba, Martínez-Manzanares Eduardo, Mancera Juan Miguel, Alarcón-López Francisco Javier, García-Márquez Jorge, Moriñigo Miguel Ángel, Arijo Salvador
Departamento de Microbiología, Facultad de Ciencias, Instituto Andaluz de Biotecnología y Desarrollo Azul (IBYDA), Universidad de Málaga, Ceimar-Universidad de Málaga, Campus Universitario de Teatinos s/n, 29071 Málaga, Spain.
Departamento de Biología y Geología, Escuela Superior de Ingeniería, Universidad de Almería, Ceimar-Universidad de Almería, 04120 La Cañada de San Urbano, Spain.
Animals (Basel). 2025 Jul 5;15(13):1982. doi: 10.3390/ani15131982.
The use of postbiotics for dietary fortification in aquaculture is gaining increasing attention due to their potential immunomodulatory and gut health benefits. In this study, we evaluated the effects of postbiotics derived from DCF12.2 on intestinal histology, microbiota composition, and the expression of genes related to epithelial integrity and inflammation in juvenile gilthead seabream (). Fish were fed either a control (CRTL) diet or the postbiotic-supplemented diet (VP) for 62 days. At the end of the feeding trial, a lipopolysaccharide (LPS) challenge was conducted to evaluate the immune response in fish. Histological analysis revealed a healthy mucosa in both groups, though fish fed the VP diet reduced fold height and mucosal layer thickness, alongside a significant increase in goblet cells. Microbiota profiling indicated higher alpha diversity and significant shifts in community composition in the VP group, including enrichment of potentially beneficial genera () and depletion of opportunistic taxa (). After the feeding trial, fish fed the VP diet exhibited downregulation of pro-inflammatory markers (). Following LPS challenge, -a key epithelial adhesion protein required for maintaining intestinal barrier integrity-expression was upregulated significantly in the VP group, suggesting enhanced epithelial resilience. These findings demonstrate that dietary fortification with -derived postbiotics supports mucosal health as well as modulates the intestinal microbiota and immune responses in gilthead seabream juveniles, offering a promising strategy for functional aquafeed development in sustainable aquaculture.
由于后生元具有潜在的免疫调节和肠道健康益处，其在水产养殖中的膳食强化应用正日益受到关注。在本研究中，我们评估了源自DCF12.2的后生元对金头鲷幼鱼肠道组织学、微生物群组成以及与上皮完整性和炎症相关基因表达的影响。将鱼分为两组，分别投喂对照（CRTL）饲料或添加后生元的饲料（VP），持续62天。在饲养试验结束时，进行脂多糖（LPS）刺激以评估鱼的免疫反应。组织学分析显示两组鱼的黏膜均健康，不过投喂VP饲料的鱼褶皱高度和黏膜层厚度降低，同时杯状细胞显著增加。微生物群分析表明，VP组的α多样性更高，群落组成发生显著变化，包括潜在有益菌属的富集和机会性类群的减少。饲养试验后，投喂VP饲料的鱼促炎标志物表达下调。LPS刺激后，维持肠道屏障完整性所需的关键上皮粘附蛋白——在VP组中显著上调，表明上皮弹性增强。这些发现表明，用源自DCF12.2的后生元进行膳食强化可支持金头鲷幼鱼的黏膜健康，并调节其肠道微生物群和免疫反应，为可持续水产养殖中功能性水产饲料的开发提供了一种有前景的策略。
