利用nMatch质谱法高效检测N-糖组学中的N-唾液酸聚糖
Efficient Detection of N-sialoglycans in N-glycomics Using Mass Spectrometry by nMatch.
作者信息
Zhao Shanshan, Du Shouwen, Guan Yudong, Schlüter Hartmut
机构信息
Department of Critical Care Medicine, Guangdong Provincial Clinical Research Center for Geriatrics, Shenzhen Clinical Research Center for Geriatrics, Shenzhen People's Hospital, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, 518020, Guangdong, China.
Section Mass Spectrometry and Proteomics, Center for Diagnostics, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany.
出版信息
Appl Biochem Biotechnol. 2025 Jul 15. doi: 10.1007/s12010-025-05337-7.
The structural instability and electronegativity of sialic acids make N-sialoglycan profiling challenging. We compared commonly used preparation strategies, including native, acetohydrazide-based derivatization, and permethylation, in N-glycomics with nMatch, an R-based bioinformatics tool that ensures full coverage of the detected N-glycans using mass spectrometry. We demonstrated that permethylation leads to efficient detection of not only N-sialoglycans but also other N-glycans and, therefore, provides a valuable reference for the selection of the appropriate N-glycan preparation strategy in future studies.
唾液酸的结构不稳定性和电负性使得N-唾液酸聚糖分析具有挑战性。我们使用nMatch（一种基于R的生物信息学工具，可确保通过质谱法全面覆盖检测到的N-聚糖），比较了N-糖组学中常用的制备策略，包括天然、基于乙酰肼的衍生化和全甲基化。我们证明，全甲基化不仅能有效检测N-唾液酸聚糖，还能检测其他N-聚糖，因此为未来研究中选择合适的N-聚糖制备策略提供了有价值的参考。
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