Pape E H, Klott K, Kreutz W
Biophys J. 1977 Aug;19(2):141-61. doi: 10.1016/S0006-3495(77)85576-8.
Diffraction patterns of stacked hemolyzed erythrocyte ghosts in the wet state were recorded. Three orders of a surprisingly high first-order periodicity of 600 A were detected. The scattering curves were evaluated by the Q-function method, including lattice distortions of the one-dimensional multilamellar system. The resulting electron density profile of the membrane in the wet state is strongly asymmetric. It consists of an asymmetrical bilayer-type part and an excess of positive relative electron density at the inner, cytoplasmic, side of the membrane. The extension of the whole membrane profile in the wet state is 100--120 A. We suggest that the innermost positive density peak mainly represents the loosely bound protein components spectrin and actin, located at the cytoplasmic side of the membrane and sometimes seen as "fuzzy" material on electron micrographs.
记录了湿态下堆叠的溶血红细胞膜的衍射图样。检测到令人惊讶的高达600埃的一级周期性的三个级次。通过Q函数方法评估散射曲线，包括一维多层系统的晶格畸变。湿态下膜的所得电子密度分布强烈不对称。它由不对称的双层型部分和膜内侧（细胞质侧）过量的正相对电子密度组成。湿态下整个膜分布的延伸为100 - 120埃。我们认为最内层的正密度峰主要代表松散结合的蛋白质成分血影蛋白和肌动蛋白，它们位于膜的细胞质侧，有时在电子显微镜照片上被视为“模糊”物质。
