Zulian Verdiana, Pauciullo Silvia, Colombo Daniele, McKay-Chopin Sandrine, Sias Catia, Del Nonno Franca, Scarabello Alessandra, Garbuglia Anna Rosa, Gheit Tarik
Laboratory of Virology, National Institute for Infectious Diseases "Lazzaro Spallanzani" (IRCCS), Rome, Italy.
Pathology Unit, National Institute for Infectious Diseases "Lazzaro Spallanzani" (IRCCS), Rome, Italy.
J Med Virol. 2025 Aug;97(8):e70518. doi: 10.1002/jmv.70518.
This study investigates the prevalence of beta (β) and gamma (γ) human papillomavirus (HPV) types in cervical and anal samples from women living with HIV and examines their association with alpha (α) HPV and cytological lesions. Cervical and anal swabs were collected from 138 women. Detection of β- and γ-HPV types were performed using bead-based assays, and data analysis included only samples with adequate DNA quality, as confirmed by β-globin amplification. The analysis revealed higher rates of β-HPV in anal samples (51.7%) compared to cervical samples (30.3%), with β-1 species being predominant in both sites. Furthermore, γ-HPV types were more prevalent in anal samples (36.5%) than in cervical site (16.9%), with γ-10 being the most frequently detected species. Concordance between cervical and anal samples for specific β-HPV types (κ = 0.20, 95% CI: 0.03-0.36) and γ-HPV types (κ = 0.17, 95% CI: 0.003-0.36) was poor. Co-infections with both β- and γ-HPV types in cervical samples was significantly associated with co-infection at the anal site (OR 7.7; 95% CI: 1.44-38.25; p = 0.01). There was no statistically significant association (p > 0.05) between β-HPV positivity and neoplastic precancerous lesions (LSIL or HSIL) at either the cervical or anal site. Similarly, γ-HPV positivity showed no significant association (p > 0.05) with cytological abnormalities in cervical or anal samples. In conclusion, while the prevalence of β- and γ-HPV types is higher in the anal region among HIV-positive women, the absence of a strong association with neoplastic lesions underscores their limited oncogenic potential compared to high-risk α-HPV types.
本研究调查了感染艾滋病毒女性的宫颈和肛门样本中β（β）型和γ（γ）型人乳头瘤病毒（HPV）的流行情况，并研究了它们与α（α）型HPV及细胞学病变的关联。收集了138名女性的宫颈和肛门拭子。使用基于磁珠的检测方法检测β型和γ型HPV，数据分析仅纳入经β-珠蛋白扩增确认DNA质量合格的样本。分析显示，肛门样本中β型HPV的检出率（51.7%）高于宫颈样本（30.3%），β-1型在两个部位均占主导。此外，γ型HPV在肛门样本中的流行率（36.5%）高于宫颈部位（16.9%），γ-10型是最常检测到的类型。宫颈和肛门样本中特定β型HPV（κ = 0.20，95%CI：0.03 - 0.36）和γ型HPV（κ = 0.17，95%CI：0.003 - 0.36）的一致性较差。宫颈样本中β型和γ型HPV的合并感染与肛门部位的合并感染显著相关（OR 7.7；95%CI：1.44 - 38.25；p = 0.01）。β型HPV阳性与宫颈或肛门部位的肿瘤性癌前病变（低度鳞状上皮内病变或高度鳞状上皮内病变）之间无统计学显著关联（p > 0.05）。同样，γ型HPV阳性与宫颈或肛门样本中的细胞学异常无显著关联（p > 0.05）。总之，虽然艾滋病毒阳性女性肛门区域中β型和γ型HPV的流行率较高，但与肿瘤性病变缺乏强关联，这突出了它们与高危α型HPV相比致癌潜力有限。
