Rein Kathleen S, Colon Ricardo, Romagosa Carlos R, Ohnikian Nicholas R, Francis Kirstie T, Rein Samuel R
Department of Marine and Earth Science, The Water School, Florida Gulf Coast University, Fort Myers, FL 33965, USA.
Department of Chemistry and Biochemistry, Florida International University, Miami, FL 33199, USA.
Mar Drugs. 2025 Jul 17;23(7):291. doi: 10.3390/md23070291.
To identify differentially abundant polyketide synthases (PKSs) and to characterize the biochemical consequences of brevetoxin biosynthesis, bottom-up, TMT-based quantitative proteomics and redox proteomics were conducted to compare two strains of the Florida red tide dinoflagellate , which differ significantly in their brevetoxin content. Forty-eight PKS enzymes potentially linked to brevetoxin production were identified, with thirty-eight showing up to 16-fold higher abundance in the high-toxin strain. A pronounced shift toward a more oxidized redox state was observed in this strain's proteome. Notably, 25 antioxidant-related proteins were significantly elevated, including alternative oxidase (AOX), which increased by 17-fold. These results elucidate the cellular consequences of toxin biosynthesis in , offer new leads for the study of brevetoxin biosynthesis, and suggest a novel red tide mitigation approach targeting high toxin-producing strains.
为了鉴定差异丰富的聚酮合酶（PKSs）并表征短裸甲藻毒素生物合成的生化后果，采用自下而上的基于TMT的定量蛋白质组学和氧化还原蛋白质组学方法，比较了两株佛罗里达红潮甲藻，它们的短裸甲藻毒素含量差异显著。鉴定出48种可能与短裸甲藻毒素产生相关的PKS酶，其中38种在高毒素菌株中的丰度高达16倍。在该菌株的蛋白质组中观察到明显向更氧化的氧化还原状态转变。值得注意的是，25种抗氧化相关蛋白显著升高，包括交替氧化酶（AOX），其增加了17倍。这些结果阐明了毒素生物合成在细胞中的后果，为短裸甲藻毒素生物合成的研究提供了新线索，并提出了一种针对高毒素产生菌株的新型赤潮缓解方法。
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