Saucedo-Montalvo Mario C, Davila-Barboza Jesus A, Gutierrez-Rodriguez Selene M, Lopez-Monroy Beatriz, Favela-Lara Susana, Rodriguez-Sanchez Iram P, Reyes-Solis Guadalupe Del C, Bobadilla-Utrera Cristina, Flores Adriana E
Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon, Av. Universidad s/n Cd. Universitaria, San Nicolas de los Garza NL 66455, Mexico.
Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autonoma de Yucatan, Merida 97000, Yucatan, Mexico.
Trop Med Infect Dis. 2025 Jun 27;10(7):182. doi: 10.3390/tropicalmed10070182.
is a widely distributed vector of in Mexico and Central America, found across a range of habitats from sylvatic to domestic. Vector control has relied heavily on indoor residual spraying with pyrethroids; however, reinfestation and emerging resistance have limited its long-term effectiveness. In this study, we analyzed the genetic diversity and population structure of from Veracruz, Oaxaca, and Yucatan using mitochondrial markers (cyt b and ND4) and screened for knockdown resistance (kdr)-type mutations in the voltage-gated sodium channel (VGSC) gene. High haplotype diversity and regional differentiation were observed, with most genetic variation occurring between populations. The ND4 marker provided greater resolution than cyt b, revealing ten haplotypes and supporting evidence of recent population expansion. Haplotype networks showed clear geographic segregation, particularly between populations east and west of the Isthmus of Tehuantepec. The L925I mutation, highly associated with pyrethroid resistance, was detected for the first time in Mexican populations of , albeit at low frequencies. These findings highlight the importance of integrating population genetic data and resistance surveillance into regionally adapted vector control strategies for Chagas disease.
在墨西哥和中美洲是一种分布广泛的媒介，存在于从野生到家庭的一系列栖息地中。病媒控制严重依赖于使用拟除虫菊酯进行室内滞留喷洒；然而，再次侵染和新出现的抗性限制了其长期效果。在本研究中，我们使用线粒体标记（细胞色素b和ND4）分析了来自韦拉克鲁斯、瓦哈卡和尤卡坦的[某种生物]的遗传多样性和种群结构，并筛选了电压门控钠通道（VGSC）基因中的击倒抗性（kdr）型突变。观察到高单倍型多样性和区域分化，大多数遗传变异发生在种群之间。ND4标记比细胞色素b提供了更高的分辨率，揭示了十种单倍型，并支持了近期种群扩张的证据。单倍型网络显示出明显的地理隔离，特别是在特万特佩克地峡以东和以西的种群之间。与拟除虫菊酯抗性高度相关的L925I突变首次在墨西哥的[某种生物]种群中被检测到，尽管频率较低。这些发现突出了将种群遗传数据和抗性监测纳入恰加斯病区域适应性病媒控制策略的重要性。
