Nazarov Kirill, Perik-Zavodskii Roman, Shevchenko Julia, Sennikov Sergey
Laboratory of Molecular Immunology, Federal State Budgetary Scientific Institution "Research Institute of Fundamental and Clinical Immunology", 630099 Novosibirsk, Russia.
Curr Issues Mol Biol. 2025 Jul 15;47(7):550. doi: 10.3390/cimb47070550.
This study identifies the novel effects of soluble factors derived from murine erythroblasts on lymphoid cell phenotypes. These effects were observed following the treatment of splenic mononuclear cells with erythroblast-conditioned media received from both healthy mice and mice subjected to hematopoiesis-activating conditions (hypoxia, blood loss, and hemolytic anemia), suggesting a common mechanism of action. Using flow cytometry, we elucidated that erythroblast-derived soluble products modulate T cell differentiation by promoting Treg development and increasing PD-1 surface expression on B cells. The immunoregulatory potential of erythroblasts is subpopulation-dependent: CD45+ erythroblasts respond to hemolytic stress by upregulating the surface expression of immunosuppressive molecules PDL1 and Galectin-9, while CD45- erythroblasts primarily increase TGFb production. These findings highlight the regulatory role of erythroblasts in modulating immune responses.
本研究确定了源自小鼠成红细胞的可溶性因子对淋巴细胞表型的新作用。在用来自健康小鼠以及经历造血激活条件（缺氧、失血和溶血性贫血）的小鼠的成红细胞条件培养基处理脾单核细胞后，观察到了这些作用，这表明存在共同的作用机制。通过流式细胞术，我们阐明了成红细胞衍生的可溶性产物通过促进调节性T细胞（Treg）发育和增加B细胞上程序性死亡受体1（PD-1）的表面表达来调节T细胞分化。成红细胞的免疫调节潜力取决于亚群：CD45+成红细胞通过上调免疫抑制分子程序性死亡受体配体1（PDL1）和半乳糖凝集素-9（Galectin-9）的表面表达来应对溶血应激，而CD45-成红细胞主要增加转化生长因子β（TGFβ）的产生。这些发现突出了成红细胞在调节免疫反应中的调节作用。
