Xu Yuze, Stuart David T
Department of Biochemistry, University of Alberta, Edmonton, AB T6G 2H7, Canada.
Microorganisms. 2025 Jun 24;13(7):1463. doi: 10.3390/microorganisms13071463.
Mannosyl erythritol lipids (MELs) are glycolipid biosurfactants produced by yeasts. The MEL biosynthetic pathway has been characterized in where a putative transporter encoded by is required for the secretion of the glycolipid surfactant to the extracellular space. The anamorphic yeast is a prolific producer of MELs, but the mechanism of MEL secretion is less well characterized than in . Homologous recombination was employed to generate a disruption of the gene in JCM10317. This mutation did not prevent the intracellular accumulation of MEL species but did result in significantly reduced secretion of the conventional MEL-A, MEL-B and MEL-C species detectable by thin-layer chromatography. However, the mutant strain did secrete a glycolipid species that is distinct from conventional MEL-A/B/C and similar to a glycolipid secreted by mutant strains of and . Despite the defect in MEL secretion displayed by the strain harbouring a disrupted gene, these cells did not display a significant defect in growth or cell morphology. The findings of this investigation provide evidence that encodes a transporter required for the secretion of MELs but not required for MEL synthesis or cell growth.
甘露糖赤藓糖醇脂（MELs）是酵母产生的糖脂类生物表面活性剂。MEL生物合成途径已得到表征，其中由[基因名称]编码的一种假定转运蛋白是糖脂表面活性剂分泌到细胞外空间所必需的。无性型酵母[酵母名称]是MELs的高产菌株，但与[对比的酵母]相比，MEL分泌机制的特征描述较少。采用同源重组技术在[酵母名称]JCM10317中破坏[基因名称]基因。该突变并未阻止MEL种类在细胞内的积累，但确实导致通过薄层色谱法可检测到的传统MEL - A、MEL - B和MEL - C种类的分泌显著减少。然而，突变菌株确实分泌了一种糖脂种类，它不同于传统的MEL - A/B/C，且类似于[对比的酵母]的[突变菌株名称]突变菌株分泌的一种糖脂。尽管携带破坏的[基因名称]基因的[酵母名称]菌株在MEL分泌方面存在缺陷，但这些细胞在生长或细胞形态方面并未表现出明显缺陷。本研究结果提供了证据，表明[基因名称]编码一种MEL分泌所必需的转运蛋白，但不是MEL合成或细胞生长所必需的。
