Lagadec Erwan, Kahrs Ingeborg Emilie Berg, Frisch Kathleen, Duesund Henrik, Nylund Are, Småge Sverre Bang
Fish Disease Research Group, Department of Biological Sciences, University of Bergen, 5007 Bergen, Norway.
Cermaq Canada, Campbell River, BC V9W 2C2, Canada.
Microorganisms. 2025 Jul 3;13(7):1567. doi: 10.3390/microorganisms13071567.
Standard blood agar medium with 2% NaCl (BAS) and Marine Agar (MA) are commonly used in bacteriological investigations of winter ulcers in farmed Atlantic salmon ( Linnaeus) in Norway and allow easy recovery of based on its characteristic viscous colonies and β-hemolytic activity. However, the recent increase in cases of winter ulcers involving spp. and the potential emergence of due to rising temperatures highlight the need for improved methods of isolation and identification. Indeed, the recovery of spp. from outbreaks of winter ulcers or tenacibaculosis can be challenging. Despite the development of several agar media over the years to overcome this issue, such as medium (FMM), it remains difficult to differentiate species. We evaluated the growth dynamics and phenotypic characteristics of 13 bacterial isolates commonly associated with ulcer outbreaks on five different agar media, including two new formulations: kanamycin-supplemented marine blood agar for the selective isolation of spp. (KABAMA) and general blood agar for marine bacteria (BAMA). These new media facilitate the identification of spp., including , by distinguishing colonies based on their specific color, shape, and hemolytic activity.
含2%氯化钠的标准血琼脂培养基（BAS）和海生琼脂（MA）常用于挪威养殖大西洋鲑（Linnaeus）冬季溃疡的细菌学调查，基于其特征性粘性菌落和β溶血活性可轻松分离出[细菌名称未给出]。然而，近期涉及[细菌名称未给出]属的冬季溃疡病例增加，以及由于温度上升可能出现[细菌名称未给出]，凸显了改进分离和鉴定方法的必要性。确实，从冬季溃疡或弧菌病暴发中分离出[细菌名称未给出]属可能具有挑战性。尽管多年来已开发出几种琼脂培养基来克服这一问题，如[培养基名称未给出]培养基（FMM），但区分[细菌名称未给出]种仍然困难。我们评估了13种通常与溃疡暴发相关的细菌分离株在五种不同琼脂培养基上的生长动态和表型特征，包括两种新配方：用于选择性分离[细菌名称未给出]属的补充卡那霉素的海生血琼脂（KABAMA）和用于海洋细菌的普通血琼脂（BAMA）。这些新培养基通过根据菌落的特定颜色、形状和溶血活性来区分菌落，便于鉴定[细菌名称未给出]属，包括[细菌名称未给出]。
