Žák Aleš, Vecka Marek, Szitanyi Peter, Floriánková Marcela, Staňková Barbora, Uhlíková Petra, Dostálová Veronika, Burda Michal
4th Department of Medicine, 1st Faculty of Medicine, Charles University in Prague and the General University Hospital in Prague, 128 08 Prague, Czech Republic.
Institute of Clinical Chemistry and Laboratory Diagnostics, 1st Faculty of Medicine, Charles University in Prague and the General University Hospital in Prague, 128 08 Prague, Czech Republic.
Nutrients. 2025 Jul 17;17(14):2347. doi: 10.3390/nu17142347.
Dyslipidemia and distorted fatty acid (FA) metabolism are frequent biochemical abnormalities associated with anorexia nervosa (AN). Gut microbiota is supposed to play an important role in the etiopathogenesis of AN. Apart from the digestive function of bile acids (BAs), these compounds have multiple metabolic functions due to the activation of specific receptors.
OBJECTIVE/AIMS: The aims of the study were to investigate biochemical measures, including plasma lipids (lipoproteins, respectively), fatty acid (FA) patterns, and the profile of plasma Bas, in AN patients and healthy controls (CON).
Plasma phospholipid FA and BAs profiles were analyzed in 39 women with a restrictive type of AN (AN-R; median age 17 years) and in 35 CON women (median age 20 years).
Compared to CON, AN had an increased concentration of HDL-C, increased content of palmitic acid, and decreased proportion of linoleic acid. Moreover, AN had a drop in the level of the sum of PUFA-6 and increased delta 9 desaturase activity for stearic acid. In AN, we found decreased levels of plasma tauroursodeoxycholic acid (TUDCA). In AN, concentrations of 22:5-6, 16:0, 20:3-6 and fat mass index were predic-tors of HDL-C levels (R = 0.43).
Patients with AN-R had an increased concentration of HDL-C, decreased levels of total PUFA -6, and increased activity of D9D for stearic acid. Furthermore, AN exerted decreased levels of TUDCA. Therefore, a decreased level of TUDCA could potentially serve as a marker of AN.
血脂异常和脂肪酸（FA）代谢紊乱是神经性厌食症（AN）常见的生化异常表现。肠道微生物群被认为在AN的发病机制中起重要作用。除了胆汁酸（BAs）的消化功能外，这些化合物由于特定受体的激活而具有多种代谢功能。
本研究旨在调查AN患者和健康对照（CON）的生化指标，包括血浆脂质（分别为脂蛋白）、脂肪酸（FA）模式和血浆BAs谱。
对39例限制型AN（AN-R；中位年龄17岁）女性和35例CON女性（中位年龄20岁）的血浆磷脂FA和BAs谱进行分析。
与CON相比，AN患者的高密度脂蛋白胆固醇（HDL-C）浓度升高，棕榈酸含量增加，亚油酸比例降低。此外，AN患者的多不饱和脂肪酸-6（PUFA-6）总和水平下降，硬脂酸的δ9去饱和酶活性增加。在AN患者中，我们发现血浆牛磺熊去氧胆酸（TUDCA）水平降低。在AN患者中，22:5-6、16:0、20:3-6的浓度和脂肪质量指数是HDL-C水平的预测指标（R = 0.43）。
AN-R患者的HDL-C浓度升高，总PUFA -6水平降低，硬脂酸的D9D活性增加。此外，AN患者的TUDCA水平降低。因此，TUDCA水平降低可能是AN的一个标志物。
